浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2013年
2期
95-97
,共3页
刘立宾%叶华丽%胡建成%何月龙%范大鹏
劉立賓%葉華麗%鬍建成%何月龍%範大鵬
류립빈%협화려%호건성%하월룡%범대붕
支气管肺泡灌洗液%结核杆菌%荧光定量PCR
支氣管肺泡灌洗液%結覈桿菌%熒光定量PCR
지기관폐포관세액%결핵간균%형광정량PCR
tuberculosis%bronchoalveolar lavage fluid%fluorescent quantitative PCR
目的:探讨支气管灌洗液涂片染色、支气管灌洗液培养及痰培养三种方法中结核杆菌阳性率与支气管灌洗液结核杆菌DNA拷贝数的关系.方法:对199例确诊肺结核患者分别应用荧光定量聚合酶链反应(FQ-PCR)技术检测支气管灌洗液(BALF)结核分枝杆菌DNA(TB-DNA),并按TB-DNA拷贝数分为A(1×103~)、B(1×105~)、C(≥1×107)三组,并对所有患者行灌洗液涂片染色、灌洗液培养及痰培养结核杆菌.结果:灌洗液涂片染色法A、B、C三组阳性率分别为12.0%、60.2%、92.7%,三组间阳性率比较差异有统计学意义(P<0.05),三组间两两比较差异有统计学意义(P均<0.05),且阳性率C组>B组>A组;灌洗液培养法A、B、C三组阳性率分别为18.7%、56.6%、87.8%,三组间差异有统计学意义(P<0.05),两两比较差异有统计学意义(P均<0.05),阳性率C组>B组>A组;痰培养法A、B、C三组阳性率分别为26.7%、65.1%、82.9%,三组间比较差异有统计学意义(P<0.05),两两比较差异有统计学意义,A组与B组、A组与C组比较P均<0.05,B组与C组比较P<0.05.结论:支气管灌洗液涂片染色、灌洗液培养、痰培养结核杆菌阳性率均随支气管灌洗液中结核杆菌DNA拷贝数递增而增高..
目的:探討支氣管灌洗液塗片染色、支氣管灌洗液培養及痰培養三種方法中結覈桿菌暘性率與支氣管灌洗液結覈桿菌DNA拷貝數的關繫.方法:對199例確診肺結覈患者分彆應用熒光定量聚閤酶鏈反應(FQ-PCR)技術檢測支氣管灌洗液(BALF)結覈分枝桿菌DNA(TB-DNA),併按TB-DNA拷貝數分為A(1×103~)、B(1×105~)、C(≥1×107)三組,併對所有患者行灌洗液塗片染色、灌洗液培養及痰培養結覈桿菌.結果:灌洗液塗片染色法A、B、C三組暘性率分彆為12.0%、60.2%、92.7%,三組間暘性率比較差異有統計學意義(P<0.05),三組間兩兩比較差異有統計學意義(P均<0.05),且暘性率C組>B組>A組;灌洗液培養法A、B、C三組暘性率分彆為18.7%、56.6%、87.8%,三組間差異有統計學意義(P<0.05),兩兩比較差異有統計學意義(P均<0.05),暘性率C組>B組>A組;痰培養法A、B、C三組暘性率分彆為26.7%、65.1%、82.9%,三組間比較差異有統計學意義(P<0.05),兩兩比較差異有統計學意義,A組與B組、A組與C組比較P均<0.05,B組與C組比較P<0.05.結論:支氣管灌洗液塗片染色、灌洗液培養、痰培養結覈桿菌暘性率均隨支氣管灌洗液中結覈桿菌DNA拷貝數遞增而增高..
목적:탐토지기관관세액도편염색、지기관관세액배양급담배양삼충방법중결핵간균양성솔여지기관관세액결핵간균DNA고패수적관계.방법:대199례학진폐결핵환자분별응용형광정량취합매련반응(FQ-PCR)기술검측지기관관세액(BALF)결핵분지간균DNA(TB-DNA),병안TB-DNA고패수분위A(1×103~)、B(1×105~)、C(≥1×107)삼조,병대소유환자행관세액도편염색、관세액배양급담배양결핵간균.결과:관세액도편염색법A、B、C삼조양성솔분별위12.0%、60.2%、92.7%,삼조간양성솔비교차이유통계학의의(P<0.05),삼조간량량비교차이유통계학의의(P균<0.05),차양성솔C조>B조>A조;관세액배양법A、B、C삼조양성솔분별위18.7%、56.6%、87.8%,삼조간차이유통계학의의(P<0.05),량량비교차이유통계학의의(P균<0.05),양성솔C조>B조>A조;담배양법A、B、C삼조양성솔분별위26.7%、65.1%、82.9%,삼조간비교차이유통계학의의(P<0.05),량량비교차이유통계학의의,A조여B조、A조여C조비교P균<0.05,B조여C조비교P<0.05.결론:지기관관세액도편염색、관세액배양、담배양결핵간균양성솔균수지기관관세액중결핵간균DNA고패수체증이증고..
Objective:To investigate the relationship between the copy number of tuberculosis-DNA(TB-DNA)in bronchoalveolar lavage fluid (BALF) by fluorescent quantitative PCR (FQ-PCR) and the positive rates of BALF smearing, BALF training,and sputum training. Methods: TB-DNA in BALF from 199 definite tuberculosis pa?tients was determined by FQ-PCR and then all samples were randomly divided into group A(1×103~), group B (1×105~),and group C(≥1×107).All samples were treated with TB BALF smearing,TB BALF training, and TB sputum training. Results: In TB BALF smearing, the positive rate was significantly different in group A,B and C (12.0%,60.2% and 92.7%,respectively, P<0.05), with the highest in group C and the lowest in group A. The pos?itive rate was significantly different in group A, B and C in TB BALF training (18.7%,56.6% and 87.8%, respec?tively, P<0.05) and among them the difference was significant, with the highest in group C and the lowest in group A. The same result was found in TB spurum training(positive rates of group A, B and C were 26.7%, 65.1% and 82.9%, respectively). Conclusion: The positive rates of BALF smearing, BALF training, and sputum training increased with the rise of TB-DNA copy number in BALF.
