CAJ | 학술논문

目的:探讨急性冠脉综合征(ACS)患者外周血淋巴细胞高表达 OX40L 及血清可溶性 OX40配体(sOX40L)和基质金属蛋白酶9(MMP-9)共同高表达的临床意义.方法:应用间接免疫荧光流式细胞术和酶联免疫吸附法(ELISA)分别对正常对照组40例、稳定型心绞痛(SA)40例、不稳定型心绞痛(UA)47例、急性心肌梗死(AMI)33例的外周血淋巴细胞表达 OX40L 及血清 sOX40L 水平进行检测,应用 ELISA 法检测各组血清 MMP-9(MMP-9).结果:(1)UA 组及 AMI 组外周血淋巴细胞表达 OX40L 明显较 SA 组和对照组高(P<0.01),SA 组与对照组比较差异无统计学意义(P>0.05).(2)UA 组及 AMI 组血清 sOX40L 水平明显较 SA 组和对照组高(P<0.01),而 SA 组与对照组比较差异亦无统计学意义(P>0.05).(3)UA 组及 AMI 组血清MMP-9水平明显较 SA 组和对照组高(P<0.01),SA 组与对照组比较差异无统计学意义(P>0.05),而且血清 sOX40L 水平与 MMP-9呈显著正相关(r=0.62, P<0.001).结论:ACS 患者外周血淋巴细胞表达 OX40L 及血清 sOX40L 和 MMP-9水平均明显升高;血清 sOX40L 水平与 MMP-9呈正相关;二者共同高表达提示冠状动脉斑块不稳定,可能是 ACS 活动性标志物.
목적:탐토급성관맥종합정(ACS)환자외주혈림파세포고표체 OX40L 급혈청가용성 OX40배체(sOX40L)화기질금속단백매9(MMP-9)공동고표체적림상의의.방법:응용간접면역형광류식세포술화매련면역흡부법(ELISA)분별대정상대조조40례、은정형심교통(SA)40례、불은정형심교통(UA)47례、급성심기경사(AMI)33례적외주혈림파세포표체 OX40L 급혈청 sOX40L 수평진행검측,응용 ELISA 법검측각조혈청 MMP-9(MMP-9).결과:(1)UA 조급 AMI 조외주혈림파세포표체 OX40L 명현교 SA 조화대조조고(P<0.01),SA 조여대조조비교차이무통계학의의(P>0.05).(2)UA 조급 AMI 조혈청 sOX40L 수평명현교 SA 조화대조조고(P<0.01),이 SA 조여대조조비교차이역무통계학의의(P>0.05).(3)UA 조급 AMI 조혈청MMP-9수평명현교 SA 조화대조조고(P<0.01),SA 조여대조조비교차이무통계학의의(P>0.05),이차혈청 sOX40L 수평여 MMP-9정현저정상관(r=0.62, P<0.001).결론:ACS 환자외주혈림파세포표체 OX40L 급혈청 sOX40L 화 MMP-9수평균명현승고;혈청 sOX40L 수평여 MMP-9정정상관;이자공동고표체제시관상동맥반괴불은정,가능시 ACS 활동성표지물.
Objective:To investigate clinical significance of OX40 Ligand (OX40L) on peripheral blood lymphocytes and increased coexpression of soluble OX40L (sOX40L) in serum and matrix metalloproteinase-9 (MMP-9) in patients with acute coronary syndromes.Methods:Forty control and 120 patients,including 40 with stable angina (SA),47 with unstable angina (UA) and 33 with acute myocardial infarction (AMI) enrolled in this study. Expression of OX40L on peripheral blood lymphocytes was analyzed by flow cytometry and sOX40L levels were measured by ELISA.MMP-9 in serum was determined by ELISA.Results:(1)Expression of OX40L on peripheral blood lymphocytes in UA and AMI patients were significantly higher than in SA patients and controls (P<0.01),while no significant difference was found between SA and controls(P>0.05).(2)The serum levels of sOX40L in UA and AMI patientswere significantly higher than in SA patients and controls(P<0.01).SOX40L levels in SA showed no significant increase when compared to controls. (3)MMP-9 in serum in UA and AMI patients were higher than in SA patients and controls (P<0.01),and the level of MMP-9 had a positive correlation with sOX40L level(r=0.62,P<0.001).Conclusion:Expression of OX40L on peripheral blood Lymphocytes,sOX40L and MMP-9 serum concentration are elevated in patients with ACS.MMP-9 level has a positive correlation with sOX40L in serum.It suggest that increased coexpression of serum MMP-9 and sOX40L may represent markers for coronary disease activity.