CAJ | 학술논문

目的:研究LAMP法和PCR法在阪崎肠杆菌快速检测中的差异,指导临床在阪崎肠杆菌快速检测中方法的选择.方法:针对阪崎肠杆菌的OmpA基因,设计相关特异性引物,分别建立LAMP法和PCR法两种检测方法体系对其进行检测,并对两种检测方法的灵敏度进行记录比较;分别采用两种方法对45株阪崎肠杆菌近源菌进行检测,分析比较两种方法的特异性;最后采用两种方法对60份商场奶粉样品进行检测,将检测结果与FDA检测结果进行比较分析.结果:两种检测方法的灵敏度检测结果显示:LAMP法对阪崎肠杆菌快速检测的敏感度(101 cfu/ml)明显高于PCR法(102 cfu/ml),两者比较差异有统计学意义(P<0.05);在对45株阪崎肠杆菌近源菌的检测中,显示LAMP法有5株阪崎肠杆菌出现阳性结果,而PCR法则显示非特异性条带,表明LAMP法检测的特异性明显优于PCR法.结论:LAMP法对阪崎肠杆菌快速检测有效可行、灵敏度高、特异性强,是临床进行阪崎肠杆菌快速检测的一种有效方法.
목적:연구LAMP법화PCR법재판기장간균쾌속검측중적차이,지도림상재판기장간균쾌속검측중방법적선택.방법:침대판기장간균적OmpA기인,설계상관특이성인물,분별건립LAMP법화PCR법량충검측방법체계대기진행검측,병대량충검측방법적령민도진행기록비교;분별채용량충방법대45주판기장간균근원균진행검측,분석비교량충방법적특이성;최후채용량충방법대60빈상장내분양품진행검측,장검측결과여FDA검측결과진행비교분석.결과:량충검측방법적령민도검측결과현시:LAMP법대판기장간균쾌속검측적민감도(101 cfu/ml)명현고우PCR법(102 cfu/ml),량자비교차이유통계학의의(P<0.05);재대45주판기장간균근원균적검측중,현시LAMP법유5주판기장간균출현양성결과,이PCR법칙현시비특이성조대,표명LAMP법검측적특이성명현우우PCR법.결론:LAMP법대판기장간균쾌속검측유효가행、령민도고、특이성강,시림상진행판기장간균쾌속검측적일충유효방법.
Objective:To study the differences between LAMP and PCR methods in the rapid detection of Enterobacter sakazakii,gave the choice of clinical method for rapid detection of Enterobacter sakazakii.Methods:Designed specific primers OmpA gene of Enterobacter sakazakii,used LAMP and PCR methods detection system,compared the sensitivity of the two detection method records;used the two ways to detect bacteria 45 Enterobacter sakazakiiion, analysed the specificity of the method;finally used the two methods for testing milk samples of 60 shopping centers,and compared the test’s results with the FDA test results.Results:The sensitivity of the test results of the two detection methods:the LAMP method rapid detection of Enterobacter sakazakii sensitivity (101 cfu/ml) was significantly higher than the PCR method (102 cfu/ml),there is a significant difference(P<0.05);detection of 45 bacteria Enterobacter sakazakii near the source LAMP method 5 Enterobacter sakazakii positive results,PCR laws of non-specific bands,indicating that the apparent specificity of the LAMP assay gifted PCR method.Conclusion:LAMP method is rapid detection of Enterobacter sakazakii effective and feasible,with high sensitivity and specificity,is an effective method for the rapid detection of Enterobacter sakazakii clinical.