漳州师范学院学报（自然科学版）
漳州師範學院學報（自然科學版）
장주사범학원학보（자연과학판）
JOURNAL OF ZHANGZHOU TEACHERS COLLEGE(NATURAL SCIENCE EDITION)
2012年
4期
44-49
,共6页
钴配合物%DNA%粘度测定%电化学
鈷配閤物%DNA%粘度測定%電化學
고배합물%DNA%점도측정%전화학
Co(PDA)2 complexes%DNA%viscosity%electrochemistry
采用紫外可见光谱、黏度测定以及电化学扫速研究金属钴配合物和DNA的相互作用,从而探索DNA的结构与功能的关系.红外光谱对配合产物进行表征,紫外吸收光谱显示,往配合物溶液中加入适量的鲱鱼精DNA后,配合物的特征吸收峰显著减弱,表明两者发生了相互作用,产生了明显的减色效应.粘度测定配合物和DNA的粘度明显增加,证明了钴配物与DNA为插入结合.电化学研究法表明钴配合物溶液与DNA作用后,复合物扩散系数明显降低,这是钴配合物溶液与DNA反应使峰电流降低.
採用紫外可見光譜、黏度測定以及電化學掃速研究金屬鈷配閤物和DNA的相互作用,從而探索DNA的結構與功能的關繫.紅外光譜對配閤產物進行錶徵,紫外吸收光譜顯示,往配閤物溶液中加入適量的鯡魚精DNA後,配閤物的特徵吸收峰顯著減弱,錶明兩者髮生瞭相互作用,產生瞭明顯的減色效應.粘度測定配閤物和DNA的粘度明顯增加,證明瞭鈷配物與DNA為插入結閤.電化學研究法錶明鈷配閤物溶液與DNA作用後,複閤物擴散繫數明顯降低,這是鈷配閤物溶液與DNA反應使峰電流降低.
채용자외가견광보、점도측정이급전화학소속연구금속고배합물화DNA적상호작용,종이탐색DNA적결구여공능적관계.홍외광보대배합산물진행표정,자외흡수광보현시,왕배합물용액중가입괄량적비어정DNA후,배합물적특정흡수봉현저감약,표명량자발생료상호작용,산생료명현적감색효응.점도측정배합물화DNA적점도명현증가,증명료고배물여DNA위삽입결합.전화학연구법표명고배합물용액여DNA작용후,복합물확산계수명현강저,저시고배합물용액여DNA반응사봉전류강저.
In this paper, the interactions between cobalt complexes and DNA have been studied and characterized by IR, UV-Vis spectra, viscosity measurement and electrochemical method. UV-Vis experimental results indicated that after adding DNA, there was an interaction between DNA and Co (PDA) 2 complexes, as the UV-Vis absorption intensity of the peaks was weakening, causing the hypochromic effect. In addition, viscosity measurement showed that the interaction between the complexes and DNA was intercalation. It was revealed by the electrochemical method that, after the interactions between the complexes and DNA, the electric current peak value was reduced, resulting in the decrease of diffusion index.
