CAJ | 학술논문

探讨了茯砖茶水提物对小鼠肠道免疫功能的调整作用.试验以注射用氨苄青霉素灌胃建立小鼠肠道菌群失调及免疫功能紊乱模型.昆明小鼠随机分6组,正常对照组、自然恢复组、模型组和茯砖茶高、中、低剂量组,分别进行肠道菌群检测、小肠黏液 sIgA 的测定、血清中 IL-2的测定、血清白蛋白和总蛋白含量检测以及回肠切片病理检测.结果表明,注射用氨苄青霉素灌胃5 d 小鼠,肠道菌群失调,小肠黏液 sIgA、血清中 IL-2、血清白蛋白和总蛋白均较正常组降低,回肠组织切片明显病变.分别灌服茯砖茶水提物后,肠道菌群得以调整,小肠黏液 sIgA、血清中 IL-2、血清白蛋白和总蛋白均有所升高,且与自然恢复组比具有显著性差异.各治疗组之间比较,高剂量组疗效优于低剂量组.说明茯砖茶水提物能修复受损黏膜,有调节肠道免疫功能的作用.
탐토료복전다수제물대소서장도면역공능적조정작용.시험이주사용안변청매소관위건립소서장도균군실조급면역공능문란모형.곤명소서수궤분6조,정상대조조、자연회복조、모형조화복전다고、중、저제량조,분별진행장도균군검측、소장점액 sIgA 적측정、혈청중 IL-2적측정、혈청백단백화총단백함량검측이급회장절편병리검측.결과표명,주사용안변청매소관위5 d 소서,장도균군실조,소장점액 sIgA、혈청중 IL-2、혈청백단백화총단백균교정상조강저,회장조직절편명현병변.분별관복복전다수제물후,장도균군득이조정,소장점액 sIgA、혈청중 IL-2、혈청백단백화총단백균유소승고,차여자연회복조비구유현저성차이.각치료조지간비교,고제량조료효우우저제량조.설명복전다수제물능수복수손점막,유조절장도면역공능적작용.
The aim of this study was to investigate the effects of FU brick tea water extracts on the adjustment of intestinal immune function of mice. Lavage with the Ampicillin to establish mice intestinal dysbacteriosis and immune dysfunction model. KM mice were random divided into six groups: normal control, natural recovery group, model group, three dose group of brick tea. The intestinal flora detection, the small intestinal mucous sIgA measurement, the measurement of IL-2 in serum, Serum albumin and total protein content detection and Ileum section pathological detection. The results showed that lavage with the Ampicillin 5 d, alteration of intestinal flora, the small intestinal mucous sIgA, the IL-2 in serum, serum albumin and total protein content were declined significantly compared with the normal saline group, in addition, the Ileum tissue slice showed obvious lesions. Compared with the natural recovery group, the small intestinal mucous sIgA, the IL-2 in serum, serum albumin and total protein content were increased significantly after the treatment with the brick tea water extracts. In the comparison between the treatment groups, the effect of high doses group were much better than the low doses group of treatment. It was concluded that the FU brick tea extracts can repair the damaged mucous membrane, showed the adjustment of the intestinal immune functions.