CAJ | 학술논문

建立了高效液相色谱/光化学反应器/荧光检测器测定茶叶中黄曲霉毒素 B1的方法.用乙腈水溶液(V∶V=86∶14)提取黄曲霉毒素 B1,提取液经净化柱和黄曲霉毒素 B1免疫亲和柱净化,高效液相色谱测定.在黄曲霉毒素 B1标准溶液质量浓度为0.591~5.91μg/L 时,峰面积与浓度呈现良好的线性关系,黄曲霉毒素 B1的回收率为85.4%~98.9%(添加量分别为0.510μg/kg、7.090μg/kg 和14.180μg/kg),相对标准偏差为0.2%~1.8%,方法检出限为0.1μg/kg.运用所建立方法对市售的8个茶样及加标样品中的黄曲霉毒素 B1进行检测,结果显示该方法选择性强、灵敏度高,适合茶叶中黄曲霉毒素 B1的测定.
건립료고효액상색보/광화학반응기/형광검측기측정다협중황곡매독소 B1적방법.용을정수용액(V∶V=86∶14)제취황곡매독소 B1,제취액경정화주화황곡매독소 B1면역친화주정화,고효액상색보측정.재황곡매독소 B1표준용액질량농도위0.591~5.91μg/L 시,봉면적여농도정현량호적선성관계,황곡매독소 B1적회수솔위85.4%~98.9%(첨가량분별위0.510μg/kg、7.090μg/kg 화14.180μg/kg),상대표준편차위0.2%~1.8%,방법검출한위0.1μg/kg.운용소건립방법대시수적8개다양급가표양품중적황곡매독소 B1진행검측,결과현시해방법선택성강、령민도고,괄합다협중황곡매독소 B1적측정.
A new method for the sensitive determination of Aflatoxins B1 in tea by high performance liquid chromatography with photoelectric reactor and fluorescence detector was established. A solution of V(acetonitrile)∶V(H2O)=86∶14 was used to extract Aflatoxins B1 from tea. The extracted solution was then purified by a multifunctional and immuneaffinity column, respectively. The peak area and the concentration of Aflatoxins B1 showed a good linear relationship within the range from 0.591 μg/L to 5.91 μg/L with a linear correlation coefficient (r) of 0.9994. The recoveries at the concentrations studied [low level (7.090 μg/kg), high level (14.180 μg/kg)] were between 85.6% and 98.9% with a relative standard deviations ranging from 1.7% to 1.8%. The limit of detection (LOD) is 0.1 μg/kg (S/N=3). The limit of quantitation (LOQ) is (0.591 μg/kg). The new method was used to analyze eight tea samples collected from the local markets and negative results were obtained. The method is suitable for detection of Aflatoxins B1 in tea with high selectivity and sensitivity.