CAJ | 학술논문

[摘要]:目的:建立美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定方法.方法:采用苯酚-硫酸法测定粘多糖的含量,采用正交试验设计考察ML-D中粘多糖的水解工艺和显色条件,采用单因素试验考察加热显色时间、苯酚和硫酸的用量.结果:ML-D中粘多糖的最佳水解工艺为:盐酸浓度6 mol/L,固液比为1∶1,水解时间1 h;最佳显色条件:苯酚2 mL,硫酸20 mL,加热显色时间10 min.结论:该法操作简便,精密度高,重复性好,可作为美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖含量的测定方法.
[적요]:목적:건립미주대렴항간섬유화활성제취물ML-D중점다당적함량측정방법.방법:채용분분-류산법측정점다당적함량,채용정교시험설계고찰ML-D중점다당적수해공예화현색조건,채용단인소시험고찰가열현색시간、분분화류산적용량.결과:ML-D중점다당적최가수해공예위:염산농도6 mol/L,고액비위1∶1,수해시간1 h;최가현색조건:분분2 mL,류산20 mL,가열현색시간10 min.결론:해법조작간편,정밀도고,중복성호,가작위미주대렴항간섬유화활성제취물ML-D중점다당함량적측정방법.
Objective: To establish a method for determining of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana. Methods: Phenol-vitriol colorimetry was used for determining content of glycosaminoglycan; orthogonal design L9(34)was adopted to investigate hydrolysis process of glycosaminoglycan in ML-D;single factor experiment was used to investigate time of color, dosage of phenol and vitriolic. Results: For the best hydrolysis process of glycosaminoglycan in ML-D, the density of hydrochloric acid is 6 mol/L, Solid-liquid ratio is 1:1, and time of hydrolysis is 1 h. There are the best conditions of color, the dosage of phenol and vitriolic respectively is 2 mL and 20 mL, heating time is 10 min. Conclusion: The method is simple and accurate with reproducibility;it can be used for the determining content of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana.