CAJ | 학술논문

[摘要]:目的:构建福氏志贺菌重组双歧杆菌Bb-ipaB疫苗.方法:以福氏志贺菌DNA为模板扩增福氏志贺菌ipaB基因,双酶切后克隆到pGEX-1λT质粒上,构建重组质粒pGEX-ipaB,电穿孔法将该质粒转化入Bb,构建福氏志贺菌重组Bb-ipaB疫苗.结果:成功扩增出分子量约为1711 bp的ipaB基因,双酶切证实基因成功插入pGEX-1λT中,并成功转化入双歧杆菌,构建rBb-ipaB疫苗.结论:成功构建福氏志贺菌重组rBb-ipaB疫苗,为该疫苗的进一步研究奠定了基础.
[적요]:목적:구건복씨지하균중조쌍기간균Bb-ipaB역묘.방법:이복씨지하균DNA위모판확증복씨지하균ipaB기인,쌍매절후극륭도pGEX-1λT질립상,구건중조질립pGEX-ipaB,전천공법장해질립전화입Bb,구건복씨지하균중조Bb-ipaB역묘.결과:성공확증출분자량약위1711 bp적ipaB기인,쌍매절증실기인성공삽입pGEX-1λT중,병성공전화입쌍기간균,구건rBb-ipaB역묘.결론:성공구건복씨지하균중조rBb-ipaB역묘,위해역묘적진일보연구전정료기출.
@@@@Objective:To construct the recombinant Bb-ipaB vaccine of Shigella flexneri. Methods:ipaB gene was amplified by PCR and cloned into pGEX-1λT plasmid to construct pGEX-ipaB. The recombinant plasmid was electroporated into Bifidobacteria bifidum (Bb)to construct rBb-ipaB vaccine. Results:A 1 711 bp gene of ipaB was successfully amplified by PCR and cloned into pGEX-1λT by restriction analysis, and the rBb-ipaB vaccine was successfully constructed by PCR and restriction analysis. Conclusion:The rBb-ipaB vaccine of Shigella flexneri is successfully constructed, which lays the experimental foundation of exploitation and utilization of this vaccine.