CAJ | 학술논문

目的初步探讨甘草水煎剂对体外培养的变异链球菌黏附能力的影响及其机制.方法将甘草用水煎煮制备成水煎剂,按所含甘草的不同质量浓度25、50、100 g·L-1分为A、 B、 C三组,不含甘草水煎剂的设为对照组.实验在玻璃试管中进行,分别收集每管中紧密黏附、非黏附和松散黏附的细菌,计算每组细菌的黏附率;用蒽酮法测定各组细菌合成水不溶性胞外多糖的含量.结果 A组、 B组、 C组和对照组的细菌黏附率分别为70.2%、40.1%14.2%、87.1%,各组细菌合成的水不溶性胞外多糖的质量浓度分别为0.28、0.25、0.13、0.30 g·L-1.结论甘草水煎剂能够明显抑制变异链球菌对光滑表面的黏附,该抑制作用存在剂量-效应关系,可能与其抑制细菌水不溶性胞外多糖的合成有关.
목적초보탐토감초수전제대체외배양적변이련구균점부능력적영향급기궤제.방법장감초용수전자제비성수전제,안소함감초적불동질량농도25、50、100 g·L-1분위A、 B、 C삼조,불함감초수전제적설위대조조.실험재파리시관중진행,분별수집매관중긴밀점부、비점부화송산점부적세균,계산매조세균적점부솔;용은동법측정각조세균합성수불용성포외다당적함량.결과 A조、 B조、 C조화대조조적세균점부솔분별위70.2%、40.1%14.2%、87.1%,각조세균합성적수불용성포외다당적질량농도분별위0.28、0.25、0.13、0.30 g·L-1.결론감초수전제능구명현억제변이련구균대광활표면적점부,해억제작용존재제량-효응관계,가능여기억제세균수불용성포외다당적합성유관.
@@@@Objective To determine the effects of licorice decoction on the adherence of Streptococcus mutans in vitro and the possible mechanisms. Methods Licorice decoction was from self-preparation. According to the licorice decoction’s mass concentration 25, 50 and 100 g·L-1, the experiment were divided into A, B and C groups. The group not containing decoction was set as negative control group. The experiment was performed in the glass tubes. Bacteria adhering tightly, loosely or not adhering to the glass were collected separately to determine bacteria’s adhesion rates. The quantity of water insoluble extracellular polysaccharides produced by bacteria was measured by anthrone method. Results Bacteria’s adhesion rates of A, B, C and negative control groups were 70.2%, 40.1%, 14.2% and 87.1% respectively. Water insoluble extracellular polysaccharides’s mass concentration of each group was 0.28, 0.25, 0.13, 0.30 g·L -1. Conclusion Licorice decoction has inhibitory potential against the adherence of Streptococcus mutans to smooth surface. The inhibition was dose-dependent, and may be relevant with the inhibition of synthesis of water insoluble extracellular polysaccharides.