CAJ | 학술논문

目的评价微振动对人脐静脉血管内皮细胞(HUVEC)增殖和分化的影响.方法将HUVEC随机分成3组, A组为空白对照, B组振动频率80 Hz,每天30 min, C组振动频率40 Hz,每天30 min,以第1天,第3天,第5天,第7天为时间点检测HUVEC的增殖能力,采用噻唑蓝法检测细胞增殖并绘制生长曲线.根据生长曲线的结果,使用增殖能力最强一组的微振动参数设定,另取HUVEC分为4组, a组接种后即刻予以振动处理, b组为a组的空白对照, c组于接种后予以振动处理3 d, d组为c组的空白对照,蛋白质印迹法检测细胞间黏附蛋白(ICAM)-1、内皮型一氧化氮合酶(eNOS)和磷酸化eNOS的表达情况.结果观察HUVEC生长曲线可见,前3 d为增长期,第5天开始进入平台期. C组细胞的增殖能力从第1天起即高于B组,第3天时高于A组, B组细胞增殖能力始终较A组低,各组之间及各组内的光密度值的差异均有统计学意义(P<0.05). c组中ICAM-1和磷酸化-eNOS表达最高.结论低幅度的高频微振动能够促进ICAM-1和磷酸化-eNOS的表达,增强细胞黏附力.
목적평개미진동대인제정맥혈관내피세포(HUVEC)증식화분화적영향.방법장HUVEC수궤분성3조, A조위공백대조, B조진동빈솔80 Hz,매천30 min, C조진동빈솔40 Hz,매천30 min,이제1천,제3천,제5천,제7천위시간점검측HUVEC적증식능력,채용새서람법검측세포증식병회제생장곡선.근거생장곡선적결과,사용증식능력최강일조적미진동삼수설정,령취HUVEC분위4조, a조접충후즉각여이진동처리, b조위a조적공백대조, c조우접충후여이진동처리3 d, d조위c조적공백대조,단백질인적법검측세포간점부단백(ICAM)-1、내피형일양화담합매(eNOS)화린산화eNOS적표체정황.결과관찰HUVEC생장곡선가견,전3 d위증장기,제5천개시진입평태기. C조세포적증식능력종제1천기즉고우B조,제3천시고우A조, B조세포증식능력시종교A조저,각조지간급각조내적광밀도치적차이균유통계학의의(P<0.05). c조중ICAM-1화린산화-eNOS표체최고.결론저폭도적고빈미진동능구촉진ICAM-1화린산화-eNOS적표체,증강세포점부력.
@@@@Objective To evaluate the proliferation and differentiation of human umbilical vein endothelial cell (HUVEC) under microvibration. Methods HUVECs were randomly divided into three groups: Group A was blank control group, group B was vibrated under 80 Hz, 30 min per day, group C was vibrated under 40 Hz, 30 min per day; observed HUVEC proliferations based on micro-vibration duration on 0, 3, 5, 7 d. Methyl thiazolyl tetra-zolium(MTT) assay was used to detect cell proliferation and growth curve. According to the result of the growth curve, we took another HUVECs divided into 4 groups: Group a was vibrated immediately, group b was blank control of group a, group c was vibrated during three days, group d was blank control of group c. Western blot assay was used to detect secretion of intercellular adhesion molecule(ICAM)-1, endothelial nitric oxide synthase (eNOS) and phosphorylate-eNOS. Results MTT growth curve showed the proliferation mainly from the first to the third day, stepped into the plateau period from the fifth day. Group C proliferated more quickly than in group B from the first day, more quickly than group A from the third day, proliferation capacity of group B was always lower than group A, and the differences were statistically significant(P<0.05). The expressions of ICAM-1 and P-eNOS in group c were the highest in all groups. Conclusion The low amplitude high frequency microvibration promoted ICAM-1 and P-eNOS expression, enhanced cell adhesion.