CAJ | 학술논문

目的:观察肥胖倾向型小鼠附睾脂肪垫中β3肾上腺素能受体(β3-AR)mRNA水平.方法:通过高脂饮食诱导肥胖倾向小鼠模型,正常饮食组作为对照(n=8),高脂饮食组(n=8)饲喂5周,鉴定为肥胖倾向型小鼠,获得附睾脂肪垫组织.逆转录和半定量聚合酶链反应(SQ-PCR)法分析β3-AR mRNA的表达,GAPDH mRNA为内参照.结果:肥胖倾向型和非倾向型小鼠脂肪垫β3-AR/GAPDH mRNA比分别为1.98±0.34和1.19±0.26,肥胖倾向型小鼠附睾脂肪垫β3-AR mRNA的表达水平上调具有统计学意义(P<0.01).结论:β3-AR与脂肪代谢密切相关,可望作为治疗干预人类肥胖和Ⅱ型糖尿病的潜在靶点.
목적:관찰비반경향형소서부고지방점중β3신상선소능수체(β3-AR)mRNA수평.방법:통과고지음식유도비반경향소서모형,정상음식조작위대조(n=8),고지음식조(n=8)사위5주,감정위비반경향형소서,획득부고지방점조직.역전록화반정량취합매련반응(SQ-PCR)법분석β3-AR mRNA적표체,GAPDH mRNA위내삼조.결과:비반경향형화비경향형소서지방점β3-AR/GAPDH mRNA비분별위1.98±0.34화1.19±0.26,비반경향형소서부고지방점β3-AR mRNA적표체수평상조구유통계학의의(P<0.01).결론:β3-AR여지방대사밀절상관,가망작위치료간예인류비반화Ⅱ형당뇨병적잠재파점.
Objective: To detect the level of β3-adrenergic receptor (β3-AR) mRNA in epididymal fat pad of lipophilic mice. Methods: Lipophilic mice (n=8) were induced via a high-fat diet. Controlled mice (n=8) fed the standard rodent diet. After five weeks feeding, epididymal fat pads were obtained. The expression of β3-AR mRNA was analyzed by using reverse transcription and semi-quantitative polymerase chain reaction (SQ-PCR) with GAPDH mRNA as internal reference. Results: The fat β3-AR/GAPDH mRNA ratios were 1.98±0.34 and 1.19 ±0.26 in lipophilic group and control group respectively. The expression level of β3-AR mRNA in epididymal fat pad was significantly increased (P<0.01) in lipophilic mice. Conclusion: This result suggests thatβ3-AR mRNA expression was closely related to fat metabolism, which suggested that β3-AR was a potential target for therapeutic intervention in human obesity and type 2 diabetes.