CAJ | 학술논문

目的:探讨 miRNA-218对前列腺癌 PC3细胞的生长调控作用并对其靶基因进行预测和初步鉴定.方法:将合成的 miRNA-218转染 PC3细胞,MTT 法和平板克隆形成试验检测细胞增殖抑制率;RT-PCR 和 Western blot 方法对经TargetScan生物信息学工具预测得到的miRNA-218的靶基因TPD52进行鉴定.结果:MTT和平板克隆形成试验结果显示miRNA-218显著抑制了PC3细胞的增殖(P<0.05);生物信息学预测结合文献查阅提示TPD52可能是miRNA-218的靶基因;RT-PCR和 Western blot结果显示miRNA-218对PC3细胞中TPD52的mRNA无明显调控作用,但能够显著下调其蛋白水平.结论:TPD52可能是miRNA-218的一个新的靶基因,miRNA-218通过抑制TPD52的表达发挥抑制PC3细胞的生长作用.
목적:탐토 miRNA-218대전렬선암 PC3세포적생장조공작용병대기파기인진행예측화초보감정.방법:장합성적 miRNA-218전염 PC3세포,MTT 법화평판극륭형성시험검측세포증식억제솔;RT-PCR 화 Western blot 방법대경TargetScan생물신식학공구예측득도적miRNA-218적파기인TPD52진행감정.결과:MTT화평판극륭형성시험결과현시miRNA-218현저억제료PC3세포적증식(P<0.05);생물신식학예측결합문헌사열제시TPD52가능시miRNA-218적파기인;RT-PCR화 Western blot결과현시miRNA-218대PC3세포중TPD52적mRNA무명현조공작용,단능구현저하조기단백수평.결론:TPD52가능시miRNA-218적일개신적파기인,miRNA-218통과억제TPD52적표체발휘억제PC3세포적생장작용.
Objective:To investigate the effect of miRNA-218 on growth of PC3 cells and predict and identify the possible target. Methods: After transfection, effects of miRNA-218 on PC3 cells were examined by using MTT and colony formation assay; the target gene of miRNA-218 was predicted then determined by RT-PCT and Western blot. Results: Compared with control group, the over-expression of miRNA-218 significantly repressed the proliferation of PC3 cells (P<0.05). Through TaroetScan prediction analysis, TPD52 was considered to be a candidate target gene of miRNA-218. The results of RT-PCR and Western blot showed that over-expressed miRNA-218 in PC3 cells robustly down-regulated the protein level of TPD52 not its miRNA,consistent with typical modulation pattern by miRNA. Conclusion: TPD52 may be a new candidate target gene of miRNA-218 which exerts its inhibitory function on PC3 cell growth through inhibition of TPD52 translation.