CAJ | 학술논문

以实验室分离的F-1菌株为出发菌株,将F-1菌株的发酵液进行了小白鼠的毒性试验.试验组小鼠生长发育良好,各个脏器的色泽与大小与对照组小鼠相比无明显差别,证明此菌株为安全菌株.为了将此菌株制备成棉籽饼脱毒菌剂应用于实际生产,对F-1菌株的产芽孢条件进行了系统的研究,采用单因素试验和正交试验的方法确定了F-1的最佳培养基组成为:蔗糖0.5%、牛肉浸膏5%、FeCl30.1%、MgSO4·7H2O 0.05%、NaH2PO4·H2O 0.03%、K2HPO40.05%、MnSO4·H2O 0.02%.在此基础上的最佳培养条件为:pH值7.2、装液量50 ml/250 ml、发酵时间48 h、接种量4%,经优化后芽孢产率达到96.5%.
이실험실분리적F-1균주위출발균주,장F-1균주적발효액진행료소백서적독성시험.시험조소서생장발육량호,각개장기적색택여대소여대조조소서상비무명현차별,증명차균주위안전균주.위료장차균주제비성면자병탈독균제응용우실제생산,대F-1균주적산아포조건진행료계통적연구,채용단인소시험화정교시험적방법학정료F-1적최가배양기조성위:자당0.5%、우육침고5%、FeCl30.1%、MgSO4·7H2O 0.05%、NaH2PO4·H2O 0.03%、K2HPO40.05%、MnSO4·H2O 0.02%.재차기출상적최가배양조건위:pH치7.2、장액량50 ml/250 ml、발효시간48 h、접충량4%,경우화후아포산솔체도96.5%.
As a mold mushroom, the toxicity test of strain F-1 was carried out. The mice that fed strain F-1 were all in good health. The results show that compared with the control, the vis?cera of the mice that fed strain F-1 had no obvious difference. So it can be proved that the F-1 is a safe strain. To prepare the detoxification agents of cottonseed cake and application in the actual production, the fermentation conditions were optimized. The optimum fermentation medi?um and optimum fermentation conditions for the produce spores of F-1 were optimized by using the single factor test and orthogonal test. The results showed that the optimum fermentation me?dium was as following: sucrose 0.5%, beef extract 5%, FeCl3 0.1%, MgSO4 · 7H2O 0.05%, NaH2PO4·H2O 0.03%, K2HPO4 0.05%, MnSO4·H2O 0.02%. For the optimum fermentation condi?tions, pH value was 7.2, volume was 50 ml/250 ml, fermentation time was 48 h and inoculation amount was 4%. After optimization, the rate of producing spore was up to 96.5%.