CAJ | 학술논문

探讨林蛙油对运动训练小鼠腓肠肌自由基代谢和超微结构的影响,为林蛙油的应用和开发提供实验依据.以ICR小鼠为研究对象,通过建立灌服林蛙油小鼠递增游泳训练和游泳力竭模型,测定小鼠力竭游泳时间、腓肠肌SOD活性、MDA含量和小鼠腓肠肌超微结构的变化.林蛙油能延长小鼠力竭游泳时间,与运动组相比,林蛙油中、高剂量组有显著差异(p<0.01).运动组SOD活性显著降低,与安静组比较,有显著差异(p<0.01);与林蛙油各剂量组比较,中、高剂量组有显著差异(p<0.05).运动组MDA的含量最高,与安静组比较,有显著异(p<0.01)与林蛙油各剂量组比较,均有显著异(p<0.01).小鼠腓肠肌组织超微结构的观察表明,运动组肌原纤维上肌节结构可辨,但呈致密状,线粒体嵴断裂,呈空化状,林蛙油组损伤程度小于运动组.林蛙油可显著提高小鼠腓肠肌组织抗自由基氧化的功能,对运动造成的腓肠肌组织超微结构的损伤有明显的保护作用,对小鼠运动能力的提高有很好的作用.
탐토림와유대운동훈련소서비장기자유기대사화초미결구적영향,위림와유적응용화개발제공실험의거.이ICR소서위연구대상,통과건립관복림와유소서체증유영훈련화유영력갈모형,측정소서력갈유영시간、비장기SOD활성、MDA함량화소서비장기초미결구적변화.림와유능연장소서력갈유영시간,여운동조상비,림와유중、고제량조유현저차이(p<0.01).운동조SOD활성현저강저,여안정조비교,유현저차이(p<0.01);여림와유각제량조비교,중、고제량조유현저차이(p<0.05).운동조MDA적함량최고,여안정조비교,유현저이(p<0.01)여림와유각제량조비교,균유현저이(p<0.01).소서비장기조직초미결구적관찰표명,운동조기원섬유상기절결구가변,단정치밀상,선립체척단렬,정공화상,림와유조손상정도소우운동조.림와유가현저제고소서비장기조직항자유기양화적공능,대운동조성적비장기조직초미결구적손상유명현적보호작용,대소서운동능력적제고유흔호적작용.
@@@@To explore the impact of Rana oil on trained mice Gastrocnemius free radical metabolism and ultrastructure, provided the experimental basis for the application and development of Rana oil. The research aimed to establish the oil Rana mice fed the incremental swimming training and exhaustive swimming model. Changes of mice swimming exhausted time, gastrocnemius muscle SOD activity, MDA content and gastrocnemius muscle ultrastructure was measured. The Rana oil can extend the swimming exhausted time of mice, compared with the trained group, Rana oil of high and medium dose group had a significant difference (p<0.01). SOD activity in the trained group significantly reduced, compared with the quiet group, had a significant difference (p<0.01);comparison with Rana oil each dose group, the high and medium dose group had a significant difference (p<0.05). The highest MDA content was in trained group, compared with quiet group was significantly different (p<0.01);with Rana oil each dose group were significantly different (p<0.01). The gastrocnemius muscle ultrastructure observation showed that the sarcomere structure on the myofibrillar of the trained group is legible, but was dense, mitochondrial cristae broken, was cavitation-like, the Rana oil group degree of injury is less than the trained group. Rana oil can significantly improve the function of anti-free radical oxidation of mouse Gastrocnemius tissue, had a significant protective effect against ultrastructural damage of the Gastrocnemius tissue caused by the movement, had a good role to improve exercise capacity in mice.