CAJ | 학술논문

采用硅胶柱层析法对有抗肿瘤活性的牛蒡根石油醚和氯仿提取物进一步提取分离,得到S-1、S-2、S-3和L-1、L-2、L-3、L-4等7个洗脱组分,MTT法比色法对这些洗脱组分进行体外抗肿瘤活性的测定.实验结果表明L1、L2对MCF-7、Hep A和S180细胞,表现出较好的细胞毒活性,在测定浓度范围内呈现出良好剂量依赖性.其IC50分别为:131.31、8.56、9.33和147.01、17.42、23.55 g/mL.
채용규효주층석법대유항종류활성적우방근석유미화록방제취물진일보제취분리,득도S-1、S-2、S-3화L-1、L-2、L-3、L-4등7개세탈조분,MTT법비색법대저사세탈조분진행체외항종류활성적측정.실험결과표명L1、L2대MCF-7、Hep A화S180세포,표현출교호적세포독활성,재측정농도범위내정현출량호제량의뢰성.기IC50분별위:131.31、8.56、9.33화147.01、17.42、23.55 g/mL.
@@@@Through the further`r separated from having anti-fumor activity of burdock root petroleum ether and chloroform extract can get 7 elution components including S-1,S-2,S-3 and L-1,L-2,L-3,L-4 and so on,by using silica gel column chromatography.The antitumor activities of these eluant components in vitro were analyzed with MTT colorimetric assay.Test result shows that L-1,L-2 react with MCF-7,HepA and S180 cell, showing good cytotoxicity and good dose-dependent in range of measurement concentration.And it's IC 50 value were 131.31,8.56,9.33 g/mL and 147.01,17.42,23.55 g/mLrespectively.