CAJ | 학술논문

提出反相高效液相色谱法测定废弃食用油脂中胆固醇的方法.试验选择了以下分析条件:25%氢氧化钾浓度;乙醇加入量为5 mL;皂化时间60 min;提取溶剂为(乙醚∶石油醚)=(1∶1,体积比);水加入量为3 mL.浓缩液经Waters C18色谱柱分离,100%甲醇洗脱,在波长203 nm进行紫外检测.胆固醇浓度在0.012 mg/mL~0.5 mg/mL范围内呈线性,相关系数为0.9997,方法检出限(3S/N)为0.005 mg/mL.方法用于废弃食用油脂中胆固醇含量的测定,相对标准偏差(n=8)分别为8.26%和5.48%,加标回收率在80.8%~98.6%.
제출반상고효액상색보법측정폐기식용유지중담고순적방법.시험선택료이하분석조건:25%경양화갑농도;을순가입량위5 mL;조화시간60 min;제취용제위(을미∶석유미)=(1∶1,체적비);수가입량위3 mL.농축액경Waters C18색보주분리,100%갑순세탈,재파장203 nm진행자외검측.담고순농도재0.012 mg/mL~0.5 mg/mL범위내정선성,상관계수위0.9997,방법검출한(3S/N)위0.005 mg/mL.방법용우폐기식용유지중담고순함량적측정,상대표준편차(n=8)분별위8.26%화5.48%,가표회수솔재80.8%~98.6%.
@@@@A method for the determination of cholesterol in waste edible oil was proposed. Analytical condition were studied and following optimized parameters were given:potassium hydroxide concentration:25%;adding ethanol:5 mL;saponification time:60 min;extraction solvent:ether and petroleum ether (1∶1, 体积比);adding water:3 mL. Waters C18 column was used as stationary phase and 100%methanol was used as mobile phase in the separation. Ultraviolet detector at 203 nm was adopted in the determination. A calibration curve was established revealing a good linearity ranges between 0.012 mg/mL to 0.5 mg/mL with a correlation coefficient of 0.999 7, the detection limit (3S/N)was 0.005 mg/mL. The proposed method was used in the determination of cholesterol in waste edible oil. RSD were 8.26%and 5.48%respectively, and values of recovery were ranged from 80.8%to 98.6%.