CAJ | 학술논문

目的:建立快速分离和测定常德特色食品中甜蜜素的液相色谱紫外检测技术.方法:根据样品特性采取不同前处理方法,在强酸条件下,利用次氯酸钠将样品中的甜蜜素转化为N,N-二氯环己胺,用正己烷萃取,在ODS-C18柱(250mm×4.6mm,5μm)上用甲醇(V)+水(V)=80+20为流动相进行分离,紫外检测器检测波长为314nm.结果:该方法在甜蜜素浓度10~100μg/mL内与色谱峰面积成良好的线性关系,回收率为89.7%~101.0%,检出限为3μg,测定结果的相对偏差小于1.0%.结论:该方法简便快速,灵敏度高,干扰少,适用于基层检测机构对各种食品样品的测定.
목적:건립쾌속분리화측정상덕특색식품중첨밀소적액상색보자외검측기술.방법:근거양품특성채취불동전처리방법,재강산조건하,이용차록산납장양품중적첨밀소전화위N,N-이록배기알,용정기완췌취,재ODS-C18주(250mm×4.6mm,5μm)상용갑순(V)+수(V)=80+20위류동상진행분리,자외검측기검측파장위314nm.결과:해방법재첨밀소농도10~100μg/mL내여색보봉면적성량호적선성관계,회수솔위89.7%~101.0%,검출한위3μg,측정결과적상대편차소우1.0%.결론:해방법간편쾌속,령민도고,간우소,괄용우기층검측궤구대각충식품양품적측정.
@@@@Objective: To establish an improved technique-HPLC-UV for the rapid detection of sodium Cyclamate in Changde specialty foods. Methods: Appropriate pretreatment was taken in the measurement of sodium cyclamate according to the character of food. Sodium cyclamate in food was determined by HPLC after being derivated to N, N-dichloride-cyclohexane with hypochlorous sodium under acidic media. The derivative was extracted and then was separated on ODS-C18 column(250mm×4.6mm,5μm), by using a mobile phase of methanol (V)-water (V)=80+20 at 1.0mL/min flow rate, then detected by UV-VIS detector at 314nm. Results: The working curves of sodium cyclamate was linear in the range of 10~100ug/mL,the recovery was 89.7%~ 101.0%, the detection limit was 3μg/kg,and RSD was below 1.0%. Conclusion:This method is simple, rapid, sensitive and of little interference. So for a county detection institution, it can be successfully applied to the measurement of cyclamate in different kinds of food.