CAJ | 학술논문

背景:近来研究发现运动可以增加内皮祖细胞数量和功能,有助于改善内皮功能.但是,运动对是否能通过上调循环内皮祖细胞数量和功能改善衰老血管弹性尚不清楚. 目的:观察规律运动是否通过影响循环内皮祖细胞数量和功能改善衰老所致血管弹性下降. 方法:对10例男性健康青年志愿者和10例健康老年志愿者予以规律运动锻炼3个月,流式细胞仪测定运动前后CD34和KDR双标阳性循环内皮祖细胞水平,乙酰化低密度脂蛋白及凝集素荧光标记方法评估体外培养内皮祖细数量,检测内皮祖细胞的迁移和增殖能力,并测定运动前后脉搏波传导速度的变化. 结果与结论:与健康青年志愿者比较,老年志愿者循环内皮祖细胞数量和功能明显下降,脉搏波传导速度减退.健康志愿者规律运动后循环内皮祖细胞水平较运动前增加,乙酰化低密度脂蛋白及凝集素双阳性内皮祖细胞数量较运动前增加,内皮祖细胞迁移和增殖能力明显增强,脉搏波传导速度下降,老年志愿者变化更为明显.循环内皮祖细胞数量及功能的增加倍数和脉搏波传导速度下降倍数呈明显正相关,多元回归分析显示乙酰化低密度脂蛋白及凝集素双阳性内皮祖细胞及增殖功能的增加倍数是脉搏波传导速度下降倍数的独立预测因素.说明规律运动可通过增加循环内皮祖细胞数量和功能改善衰老所致的血管弹性下降,提示运动调节内皮祖细胞可能是其保护衰老血管损伤的作用机制之一.
배경:근래연구발현운동가이증가내피조세포수량화공능,유조우개선내피공능.단시,운동대시부능통과상조순배내피조세포수량화공능개선쇠로혈관탄성상불청초. 목적:관찰규률운동시부통과영향순배내피조세포수량화공능개선쇠로소치혈관탄성하강. 방법:대10례남성건강청년지원자화10례건강노년지원자여이규률운동단련3개월,류식세포의측정운동전후CD34화KDR쌍표양성순배내피조세포수평,을선화저밀도지단백급응집소형광표기방법평고체외배양내피조세수량,검측내피조세포적천이화증식능력,병측정운동전후맥박파전도속도적변화. 결과여결론:여건강청년지원자비교,노년지원자순배내피조세포수량화공능명현하강,맥박파전도속도감퇴.건강지원자규률운동후순배내피조세포수평교운동전증가,을선화저밀도지단백급응집소쌍양성내피조세포수량교운동전증가,내피조세포천이화증식능력명현증강,맥박파전도속도하강,노년지원자변화경위명현.순배내피조세포수량급공능적증가배수화맥박파전도속도하강배수정명현정상관,다원회귀분석현시을선화저밀도지단백급응집소쌍양성내피조세포급증식공능적증가배수시맥박파전도속도하강배수적독립예측인소.설명규률운동가통과증가순배내피조세포수량화공능개선쇠로소치적혈관탄성하강,제시운동조절내피조세포가능시기보호쇠로혈관손상적작용궤제지일.
@@@@BACKGROUND:Recent studies have shown that exercise can increase the number and ability of endothelial progenitor cel s which is beneficial to the improvement of endothelial function. However, it is stil unclear whether exercise can improve age-related decline in arterial elasticity by enhancing number and activity of circulating endothelial progenitor cel s. @@@@OBJECTIVE: To investigate whether regular exercise-induced enhanced circulating endothelial progenitor cel s improves age-related decline in arterial elasticity in healthy men. @@@@METHODS:Effects of regular exercise on circulating endothelial progenitor cel s of 10 older and 10 young healthy men undergoing 3 months regular exercise were observed. Flow cytometry analysis was performed to evaluate the number of CD34 and KDR double-positive labeled circulating endothelial progenitor cel s before and after exercise, and acetylated low-density lipoprotein;and lectin fluorescent staining method was used to evaluate the number of cultured endothelial progenitor cel s. The migratory and proliferative activities of endothelial progenitor cel s were also evaluated. In addition, brachial-ankle pulse wave velocities of older and young sedentary healthy men were measured. @@@@RESULTS AND CONCLUSION:Compared with young healthy men, the older healthy men exhibited decreased number and activity of circulating endothelial progenitor cel s and increased brachial-ankle pulse wave velocities. Flow cytometry analysis showed that the circulating endothelial progenitor cel s positive for CD34 and KDR in the peripheral blood increased after regular exercise. The acetylated-low-density lipoprotein and lectin fluorescent staining method indicated that the cultured endothelial progenitor cel s also increased after exercise. The proliferative and migratory activities of cultured endothelial progenitor cel s were significantly higher after exercise. However, the increased number and activity of circulating endothelial progenitor cel s and decreased brachial-ankle pulse wave velocities of older sedentary healthy men were higher. There was a closely positive correlation between increased circulating endothelial progenitor cel s and decreased brachial-ankle pulse wave velocities. Multivariate analysis identified increased proliferative activity of circulating endothelial progenitor cel s as an independent predictor of decreased brachial-ankle pulse wave velocities. Regular exercise-induced enhanced number and activity of circulating endothelial progenitor cel s attenuates age-related decline in arterial elasticity, indicating that the modulation of exercise on circulating endothelial progenitor cel s may be the mechanism underlying exercise-exhibited protection against age-related vascular injury.