中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
15期
2819-2826
,共8页
寇建强%王倩倩%王昌耀%王英振
寇建彊%王倩倩%王昌耀%王英振
구건강%왕천천%왕창요%왕영진
组织构建%组织构建综述%种子细胞%软骨细胞%异体软骨细胞%胚胎干细胞%骨髓间充质干细胞%细胞培养%诱导%支架%细胞因子%微环境%省级基金
組織構建%組織構建綜述%種子細胞%軟骨細胞%異體軟骨細胞%胚胎榦細胞%骨髓間充質榦細胞%細胞培養%誘導%支架%細胞因子%微環境%省級基金
조직구건%조직구건종술%충자세포%연골세포%이체연골세포%배태간세포%골수간충질간세포%세포배양%유도%지가%세포인자%미배경%성급기금
tissue construction%tissue construction review%seed cells%chondrocytes%allogeneic chondrocytes%embryonic stem cells%bone marrow mesenchymal stem cells%cell culture%induction%scaffolds%cytokine%microenvironment%provincial grants-supported paper
背景:传统的软骨缺损的修复方法都有其局限性,组织工程技术的出现从根本上改变了“以创伤修复创伤”的传统治疗模式.
目的:总结分析目前组织工程技术修复关节软骨的研究进展.
方法:由第一作者检索1990年至2011年 PubMed数据及中国知网数据库有关应用组织工程技术修复关节软骨方面的文献.共检索中文187篇,英文211篇,最终保留49篇进入结果分析.
结果与结论:软骨组织工程的主要方法就是应用工程学和生命科学原理,在体外分离、培养、扩增所需要的种子细胞,然后将之种植于合适的生物支架材料上,将细胞支架复合体植入体内组织缺损部位,并加入一定的诱导条件,逐渐形成新的有功能的软骨组织.文章在种子细胞的选择方面重点叙述了自体软骨细胞、异体软骨细胞、胚胎干细胞、骨髓间充质干细胞的研究进展;在细胞诱导及条件培养方面重点叙述了细胞因子、细胞条件培养、转基因技术的研究进展;并对生物支架材料的选择和研究进行了相关叙述.找到最理想的种子细胞,合理联合应用细胞因子,更加真实的模拟细胞生存的微环境,基因工程安全、高效、可控转染,构建理想的支架材料,将是今后组织工程研究的重点和热点.
揹景:傳統的軟骨缺損的脩複方法都有其跼限性,組織工程技術的齣現從根本上改變瞭“以創傷脩複創傷”的傳統治療模式.
目的:總結分析目前組織工程技術脩複關節軟骨的研究進展.
方法:由第一作者檢索1990年至2011年 PubMed數據及中國知網數據庫有關應用組織工程技術脩複關節軟骨方麵的文獻.共檢索中文187篇,英文211篇,最終保留49篇進入結果分析.
結果與結論:軟骨組織工程的主要方法就是應用工程學和生命科學原理,在體外分離、培養、擴增所需要的種子細胞,然後將之種植于閤適的生物支架材料上,將細胞支架複閤體植入體內組織缺損部位,併加入一定的誘導條件,逐漸形成新的有功能的軟骨組織.文章在種子細胞的選擇方麵重點敘述瞭自體軟骨細胞、異體軟骨細胞、胚胎榦細胞、骨髓間充質榦細胞的研究進展;在細胞誘導及條件培養方麵重點敘述瞭細胞因子、細胞條件培養、轉基因技術的研究進展;併對生物支架材料的選擇和研究進行瞭相關敘述.找到最理想的種子細胞,閤理聯閤應用細胞因子,更加真實的模擬細胞生存的微環境,基因工程安全、高效、可控轉染,構建理想的支架材料,將是今後組織工程研究的重點和熱點.
배경:전통적연골결손적수복방법도유기국한성,조직공정기술적출현종근본상개변료“이창상수복창상”적전통치료모식.
목적:총결분석목전조직공정기술수복관절연골적연구진전.
방법:유제일작자검색1990년지2011년 PubMed수거급중국지망수거고유관응용조직공정기술수복관절연골방면적문헌.공검색중문187편,영문211편,최종보류49편진입결과분석.
결과여결론:연골조직공정적주요방법취시응용공정학화생명과학원리,재체외분리、배양、확증소수요적충자세포,연후장지충식우합괄적생물지가재료상,장세포지가복합체식입체내조직결손부위,병가입일정적유도조건,축점형성신적유공능적연골조직.문장재충자세포적선택방면중점서술료자체연골세포、이체연골세포、배태간세포、골수간충질간세포적연구진전;재세포유도급조건배양방면중점서술료세포인자、세포조건배양、전기인기술적연구진전;병대생물지가재료적선택화연구진행료상관서술.조도최이상적충자세포,합리연합응용세포인자,경가진실적모의세포생존적미배경,기인공정안전、고효、가공전염,구건이상적지가재료,장시금후조직공정연구적중점화열점.
@@@@BACKGROUND:The traditional methods for cartilage defect repair have their limitations. The application of tissue engineering methods to repair articular cartilage defects has shown promising prospects. @@@@OBJECTIVE: To review the progress of tissue engineering technique to repair articular cartilage at home and abroad in recent years. @@@@METHODS:PubMed and CNKI databases were searched online by the first author for papers concerning applying tissue engineering technique to repair articular cartilage defects in 1990-2011. Total y 187 Chinese papers and 211 English papers were retrieved, and final y 49 papers were included in result analysis. @@@@RESULTS AND CONCLUSION:The main method of cartilage tissue engineering is to apply the principle of engineering and life sciences to isolate, culture and proliferate needed seed cel s. Then, the cel s were implanted onto the appropriate biological scaffold, and the cytoskeleton was implanted into the defect site. Gradual y, new cartilage tissues form under some inducing conditions. In recent years, the cartilage tissue engineering research focuses on the fol owing aspects:(1) Selection of seed cel s, including autologous chondrocytes, al ogenic chondrocytes, embryonic stem cel s and bone marrow mesenchymal stem cel s;(2) Cel induction and conditional culture, including cytokines, cel culture conditions, transgenic technology;(3) Selection and research of biological scaffold materials. The fol owing aspects have become hotspots in the tissue engineering research:finding ideal seed cel s, combination with cytokines, literal y imitating microenvironment for cel survival, safety, high efficiency, and control ed transfection of gene engineering, and constructing ideal scaffold materials.
