CAJ | 학술논문

背景:研究发现应用细胞与支架材料相结合可达到较理想的牙周骨缺损修复效果. 目的:观察小型猪自体牙周膜干细胞联合羟基磷灰石生物陶瓷复合物修复牙周二类骨缺损的效果. 方法:共纳入6只贵州小型猪,用台式慢机磨除的方法建立贵州小型猪上下颌骨二类牙周骨缺损模型,骨缺损位于第三前磨牙和第四前磨牙之间,暴露第四前磨牙的近中牙根.实验组应用自体上下颌尖牙牙周膜干细胞复合羟基磷灰石生物陶瓷进行缺损修复;对照组应用单纯羟基磷灰石生物陶瓷进行修复;以未进行任何修复的作为模型组.各组在骨缺损区域表面覆盖口腔生物膜.造模后12周,取各组缺损牙周组织,通过临床观察,头颅螺旋 CT 平扫及三维重建和苏木精-伊红染色观察牙周骨组织成骨愈合情况. 结果与结论:临床观察显示实验组牙周缺损愈合最好.头颅螺旋CT见实验组缺损区骨密度与周围正常牙槽骨密度无明显差异,对照组及模型组缺损区缺损仍清晰可见,呈不完全愈合状态.苏木精-伊红染色见实验组新生牙槽骨已充满骨缺损处,建立了正常的钙化骨结构;对照组新生牙槽骨尚未充满骨缺损处,钙化程度极低;模型组可见大量的胶原纤维,缺损区仍大量空缺,未见明显新骨形成.可见牙周膜干细胞联合引导组织再生能有效提高小型猪牙周二类骨缺损愈合和牙周再生能力,修复牙周骨缺损.
배경:연구발현응용세포여지가재료상결합가체도교이상적아주골결손수복효과. 목적:관찰소형저자체아주막간세포연합간기린회석생물도자복합물수복아주이류골결손적효과. 방법:공납입6지귀주소형저,용태식만궤마제적방법건립귀주소형저상하합골이류아주골결손모형,골결손위우제삼전마아화제사전마아지간,폭로제사전마아적근중아근.실험조응용자체상하합첨아아주막간세포복합간기린회석생물도자진행결손수복;대조조응용단순간기린회석생물도자진행수복;이미진행임하수복적작위모형조.각조재골결손구역표면복개구강생물막.조모후12주,취각조결손아주조직,통과림상관찰,두로라선 CT 평소급삼유중건화소목정-이홍염색관찰아주골조직성골유합정황. 결과여결론:림상관찰현시실험조아주결손유합최호.두로라선CT견실험조결손구골밀도여주위정상아조골밀도무명현차이,대조조급모형조결손구결손잉청석가견,정불완전유합상태.소목정-이홍염색견실험조신생아조골이충만골결손처,건립료정상적개화골결구;대조조신생아조골상미충만골결손처,개화정도겁저;모형조가견대량적효원섬유,결손구잉대량공결,미견명현신골형성.가견아주막간세포연합인도조직재생능유효제고소형저아주이류골결손유합화아주재생능력,수복아주골결손.
@@@@BACKGROUND:Studies have proved that autologous periodontal ligament stem cel s combined with scaffold materials can achieve better effect on the repair of periodontal bone defects. @@@@OBJECTIVE:To observe the effect of miniature pig autologous periodontal ligament stem cel s combined with hydroxyapatite bioceramic composites in the repair of category Ⅱ periodontal bone defects. @@@@METHODS:Six Guizhou miniature pigs were included, and were used to establish the miniature pig models of upper and lower jaw category Ⅱ periodontal bone defects. The bone defects were located between the third premolar and fourth premolar, and the near root of fourth premolar was exposed. The defects in the experimental group were repaired with periodontal ligament stem cel s obtained from the autologous maxil ary and mandibular canine and combined with hydroxyapatite bioceramic;the defects in the control group were repaired with hydroxyapatite bioceramic simplely;and the model group did not repaired. The defects in each group were covered with oral biofilm. At 12 weeks after modeling, the defect periodontal tissues were obtained from each group, and then the osteogenesis healing of the periodontal bone tissue was observed through clinical observation, heal spiral CT scanning, three-dimensional reconstruction and hematoxylin-eosin staining. @@@@RESULTS AND CONCLUSION:Clinical observation showed that healing of periodontal defects in the experimental group was the best. Head spiral CT scanning showed that there was no significant difference of bone mineral density between bone defect area and surrounding normal alveolar bone, and the defects in the control group and the model group were stil clear visible in the incomplete healing state. Hematoxylin-eosin staining showed that the defect area in the experimental group was fil ed with newborn alveolar bone completely, and the normal calcified bone structure was established;in the control group, the defect area was fil ed with newborn alveolar bone incompletely with low calcification degree;a large number of col agen fibers could be seen in the model group, and the defect area was not fil ed and no new bone formed. It is visible that periodontal ligament stem cel s combined with guided tissue regeneration can effectively promote healing of category II periodontal bone defects and periodontal regeneration in miniature pigs, thus repairing periodontal bone defects.