CAJ | 학술논문

背景:目前国内外有关组织工程骨材料及构建方法的研究持续升温,而临床上如何修复大面积骨缺损仍然是研究的难点和重点. 目的:对比观察同种异体骨支架材料、β-磷酸三钙材料以及碱性成纤维细胞生长因子慢病毒载体转染羊骨髓间充质干细胞的组织工程骨对羊髂骨极限骨缺损的修复效果. 方法:体外诱导培养羊骨髓间充质干细胞至第3代时,构建碱性成纤维细胞生长因子慢病毒载体.将转染及未转染的骨髓间充质干细胞同种异体支架骨材料、单纯同种异体骨支架材料、β-磷酸三钙材料回植入羊髂骨极限缺损模型(15 mm×10 mm×10 mm),修复后4,8,12周末进行影像学、组织学及扫描电镜观察. 结果与结论:碱性成纤维细胞生长因子慢病毒转染羊骨髓间充质干细胞后的组织工程骨材料修复极限骨缺损优于未转染的细胞骨支架材料、单纯同种异体骨支架材料及β-磷酸三钙材料;未转染的细胞骨支架材料优于单纯同种异体骨支架材料与β-磷酸三钙材料组;单纯同种异体骨支架材料降解速率大于β-磷酸三钙材料.结果提示,以碱性成纤维细胞生长因子慢病毒载体转染羊骨髓间充质干细胞加载同种异体骨支架材料构建的组织工程骨符合骨修复的需求,既可以引导新骨形成,又不会妨碍骨组织的重建和塑型,具有良好的生物相容性.
배경:목전국내외유관조직공정골재료급구건방법적연구지속승온,이림상상여하수복대면적골결손잉연시연구적난점화중점. 목적:대비관찰동충이체골지가재료、β-린산삼개재료이급감성성섬유세포생장인자만병독재체전염양골수간충질간세포적조직공정골대양가골겁한골결손적수복효과. 방법:체외유도배양양골수간충질간세포지제3대시,구건감성성섬유세포생장인자만병독재체.장전염급미전염적골수간충질간세포동충이체지가골재료、단순동충이체골지가재료、β-린산삼개재료회식입양가골겁한결손모형(15 mm×10 mm×10 mm),수복후4,8,12주말진행영상학、조직학급소묘전경관찰. 결과여결론:감성성섬유세포생장인자만병독전염양골수간충질간세포후적조직공정골재료수복겁한골결손우우미전염적세포골지가재료、단순동충이체골지가재료급β-린산삼개재료;미전염적세포골지가재료우우단순동충이체골지가재료여β-린산삼개재료조;단순동충이체골지가재료강해속솔대우β-린산삼개재료.결과제시,이감성성섬유세포생장인자만병독재체전염양골수간충질간세포가재동충이체골지가재료구건적조직공정골부합골수복적수구,기가이인도신골형성,우불회방애골조직적중건화소형,구유량호적생물상용성.
@@@@BACKGROUND:Nowadays, the research regarding tissue engineering bone materials and construction methods continues to heat up, and how to repair large bone defects is stil difficult in the clinic. @@@@OBJECTIVE:To compare the effects of tissue engineering bones with al ogeneic scaffold material,β-tricalcium phosphate material and basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cel s on the repairing of critical-sized iliac defects in sheep. @@@@METHODS:The sheep bone marrow mesenchymal stem cel s were in vitro induced and cultured to the third generation, and then the basic fibroblast growth factor lentiviral vector was constructed. The al ogeneic scaffold materials with transfected and untransfected bone marrow mesenchymal stem cel s, simple al ogeneic scaffold materials andβ-tricalcium phosphate materials were implanted into the sheep model of critical-sized iliac defects (15 mm×10 mm×10 mm). Imaging, histology, and scanning electron microscopy observations were performed at 4, 8 and 12 weeks after repairing. @@@@RESULTS AND CONCLUSION:The repairing effect of al ogeneic scaffold materials with basic fibroblast growth factor lentiviral vector transfected bone marrow mesenchymal stem cel s on sheep critical-sized iliac defects was better than that of untransfected al ogeneic scaffold materials, simple al ogeneic scaffold materials andβ-tricalcium phosphate materials;the effect of untransfected al ogeneic scaffold materials was better than that of simple al ogeneic scaffold materials andβ-tricalcium phosphate materials;the degradation rate of simple al ogeneic scaffold materials was higher than that ofβ-tricalcium phosphate materials. The tissue engineering bone constructed using al ogeneic scaffold materials with basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cel s can meet the requirement of bone repairing, and it can guide the new bone formation with good biocompatibility and cannot interfere with bone tissue reconstruction and modeling.