中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
16期
2874-2882
,共9页
刘斌钰%郭敏芳%邢雁霞%马存根
劉斌鈺%郭敏芳%邢雁霞%馬存根
류빈옥%곽민방%형안하%마존근
生物材料%组织工程骨材料%煅烧骨%细胞相容性%生物相容性%毒性%骨髓间充质干细胞%浸提液%组织工程骨%省级基金
生物材料%組織工程骨材料%煅燒骨%細胞相容性%生物相容性%毒性%骨髓間充質榦細胞%浸提液%組織工程骨%省級基金
생물재료%조직공정골재료%단소골%세포상용성%생물상용성%독성%골수간충질간세포%침제액%조직공정골%성급기금
biomaterials%tissue-engineered bone materials%true bone ceramic%cellular compatibility%biocompatibility%toxicity%bone marrow mesenchymal stem cells%water extract%tissue-engineered bone%bone%provincial grants-supported paper
背景:经高温处理的煅烧骨具有类似自然骨的连续微孔结构,良好的生物相容性和降解性.
目的:观察牛煅烧骨的生物相容性、细胞相容性及毒性.
方法:①细胞相容性实验:将牛煅烧骨与第3代已诱导的Wistar大鼠骨髓间充质干细胞复合培养.②溶血实验:将煅烧骨浸提液、生理盐水与双蒸水加入兔血中.③凝血实验:将煅烧骨加入兔血浆中.④急性毒性实验:在昆明种小鼠尾静脉分别注射煅烧骨浸提液、生理盐水.⑤微核实验:在小鼠腹腔分别注射煅烧骨浸提液、生理盐水与环磷酰胺.⑥局部刺激性实验:将煅烧骨浸提液、生理盐水分别注射于兔两侧脊柱皮下.⑦热源检测实验:在兔耳静脉注射煅烧骨浸提液.⑧皮下植入实验:将煅烧骨材料植入Wistar大鼠背部皮下.
结果与结论:煅烧骨材料无细胞毒性,具有良好的细胞及血液相容性;对皮肤、肌肉无刺激作用;对心、肝、肾重要器官无毒性作用;皮下植入后对周围组织无刺激作用,能够部分降解吸收并被机体组织替代;无致热作用,对凝血功能无影响,对小鼠骨髓细胞无抑制及毒性作用.
揹景:經高溫處理的煅燒骨具有類似自然骨的連續微孔結構,良好的生物相容性和降解性.
目的:觀察牛煅燒骨的生物相容性、細胞相容性及毒性.
方法:①細胞相容性實驗:將牛煅燒骨與第3代已誘導的Wistar大鼠骨髓間充質榦細胞複閤培養.②溶血實驗:將煅燒骨浸提液、生理鹽水與雙蒸水加入兔血中.③凝血實驗:將煅燒骨加入兔血漿中.④急性毒性實驗:在昆明種小鼠尾靜脈分彆註射煅燒骨浸提液、生理鹽水.⑤微覈實驗:在小鼠腹腔分彆註射煅燒骨浸提液、生理鹽水與環燐酰胺.⑥跼部刺激性實驗:將煅燒骨浸提液、生理鹽水分彆註射于兔兩側脊柱皮下.⑦熱源檢測實驗:在兔耳靜脈註射煅燒骨浸提液.⑧皮下植入實驗:將煅燒骨材料植入Wistar大鼠揹部皮下.
結果與結論:煅燒骨材料無細胞毒性,具有良好的細胞及血液相容性;對皮膚、肌肉無刺激作用;對心、肝、腎重要器官無毒性作用;皮下植入後對週圍組織無刺激作用,能夠部分降解吸收併被機體組織替代;無緻熱作用,對凝血功能無影響,對小鼠骨髓細胞無抑製及毒性作用.
배경:경고온처리적단소골구유유사자연골적련속미공결구,량호적생물상용성화강해성.
목적:관찰우단소골적생물상용성、세포상용성급독성.
방법:①세포상용성실험:장우단소골여제3대이유도적Wistar대서골수간충질간세포복합배양.②용혈실험:장단소골침제액、생리염수여쌍증수가입토혈중.③응혈실험:장단소골가입토혈장중.④급성독성실험:재곤명충소서미정맥분별주사단소골침제액、생리염수.⑤미핵실험:재소서복강분별주사단소골침제액、생리염수여배린선알.⑥국부자격성실험:장단소골침제액、생리염수분별주사우토량측척주피하.⑦열원검측실험:재토이정맥주사단소골침제액.⑧피하식입실험:장단소골재료식입Wistar대서배부피하.
결과여결론:단소골재료무세포독성,구유량호적세포급혈액상용성;대피부、기육무자격작용;대심、간、신중요기관무독성작용;피하식입후대주위조직무자격작용,능구부분강해흡수병피궤체조직체대;무치열작용,대응혈공능무영향,대소서골수세포무억제급독성작용.
@@@@BACKGROUND:True bone ceramic treated with high temperature has continuous microporous structure, good biological compatibility and degradation, which are analogous to nature bone. @@@@OBJECTIVE:To observe the biocompatibility, cel ular compatibility and acute toxicity of implanted composite made from periosteal-derived osteoblasts and sintered bovine bone (true bone ceramics). @@@@METHODS:Experiment of cel ular compatibility:bovine true bone ceramics were compound cultured with induced passage 3 bone marrow mesenchymal stem cel s of Wistar rats. Hemolysis test:the true bone ceramic water extract, normal saline and double-distil ed water were added into rabbit blood. Blood coagulation test:the true bone ceramics were added into rabbit blood plasma. Systemic acute toxicity test:true bone ceramic water extract and normal saline were injected into the Kunming mice via the tail vein respectively. Micronucleus test:true bone ceramic water extract, normal saline and cyclophosphamide were given into the mice through intraperitoneal injection respectively. Local irritation reaction:true bone ceramic water extract and normal saline were injected at the two sides of the spine respectively. Pyrogen test:true bone ceramic water extract was injected via the ear vein. The subcutaneous implantation test:the true bone ceramic materials were implanted dorsal subcutaneously. @@@@RESULTS AND CONCLUSION:True bone ceramics had no cel toxicity, but had good cel ular and blood compatibility;had no stimulation to muscle and skin;had no toxicity on heart, liver and kidney;had no stimulation to the surrounding tissues after subcutaneous implantation, and part of the materials were degraded and replaced by newly developed fibrous tissue;had no pyrogenic action and had no influence on coagulation;had no inhibition and toxic effect on bone marrow cel s.