CAJ | 학술논문

背景:研究证明骨髓基质干细胞与煅烧骨支架材料结合后可形成组织工程化骨,但在动物体内的生物相容性及皮下诱导成骨的能力国内报道较少. 目的:观察骨髓基质细胞复合异种煅烧骨植入BALB/c裸鼠背部皮下的成骨性能及煅烧骨材料作为组织工程骨支架材料的可行性. 方法:选用经脱脂及脱蛋白处理后高温煅烧形成的骨支架材料与梯度密度离心法分离培养至第3代的羊骨髓基质干细胞构建细胞-煅烧骨复合物植入 BALB/c 裸鼠背部皮下,选同期对侧背部皮下植入单纯煅烧骨为对照组. 结果与结论:煅烧后的松质骨块为白垩色,表面呈蜂窝状多孔结构,保留了天然松质骨的多孔状空间结构.骨小梁结构完整,孔隙相互连通.骨髓基质干细胞接种到煅烧骨后24 h可见大量细胞黏附于支架上,7 d后细胞分泌大量细胞外基质,细胞与基质分界不清,细胞能在材料上良好地黏附、增殖与生长,细胞活性未受到支架材料的影响.植入4周后,两组均可见煅烧骨边缘出现少量残片,细胞-煅烧骨复合物组煅烧骨孔隙周边可发现骨细胞,对照组煅烧骨表面可见纤维结缔组织包绕.植入后8周,两组均可见到煅烧骨部分降解为片状类骨质,周围有成纤维细胞包绕,排列紧密,形态多样,细胞-煅烧骨复合物组煅烧骨孔隙内可见煅烧骨表面有排列成行的成骨细胞,孔隙间有散在淋巴细胞浸润.对照组标本可见孔隙内有大量结缔组织长入,未见明显成骨迹象.结果说明,经高温煅烧后的松质骨材料,具有良好的生物相容性和生物安全性,可作为骨髓基质干细胞的良好载体,复合后植入体内能够诱导新生骨组织形成,可作为骨缺损组织工程修复的支架材料.
배경:연구증명골수기질간세포여단소골지가재료결합후가형성조직공정화골,단재동물체내적생물상용성급피하유도성골적능력국내보도교소. 목적:관찰골수기질세포복합이충단소골식입BALB/c라서배부피하적성골성능급단소골재료작위조직공정골지가재료적가행성. 방법:선용경탈지급탈단백처리후고온단소형성적골지가재료여제도밀도리심법분리배양지제3대적양골수기질간세포구건세포-단소골복합물식입 BALB/c 라서배부피하,선동기대측배부피하식입단순단소골위대조조. 결과여결론:단소후적송질골괴위백성색,표면정봉와상다공결구,보류료천연송질골적다공상공간결구.골소량결구완정,공극상호련통.골수기질간세포접충도단소골후24 h가견대량세포점부우지가상,7 d후세포분비대량세포외기질,세포여기질분계불청,세포능재재료상량호지점부、증식여생장,세포활성미수도지가재료적영향.식입4주후,량조균가견단소골변연출현소량잔편,세포-단소골복합물조단소골공극주변가발현골세포,대조조단소골표면가견섬유결체조직포요.식입후8주,량조균가견도단소골부분강해위편상류골질,주위유성섬유세포포요,배렬긴밀,형태다양,세포-단소골복합물조단소골공극내가견단소골표면유배렬성행적성골세포,공극간유산재림파세포침윤.대조조표본가견공극내유대량결체조직장입,미견명현성골적상.결과설명,경고온단소후적송질골재료,구유량호적생물상용성화생물안전성,가작위골수기질간세포적량호재체,복합후식입체내능구유도신생골조직형성,가작위골결손조직공정수복적지가재료.
@@@@BACKGROUND:It has proved that the bone marrow stromal cel s and sintered bone support material combination can form tissue-engineered bone, but relevant studies on its biocompatibility in animals and subcutaneous osteogenesis ability are less reported in China. @@@@OBJECTIVE:To observe the osteogenesis capacity of marrow stromal stem cel s combined with sintered bone implanted into Balb/c nude mouse back subcutaneously and to explore the feasibility of sintered bone as a scaffold for tissue-engineered bone. @@@@METHODS:Sintered bone scaffold materials were prepared using defatted and deproteinized processing and high-temperature calcinations. Then the sintered bone was combined with passage 3 sheep bone marrow stromal stem cel s cultured using density gradient centrifugation method to be implanted subcutaneously into the back of BALB/c nude mice. Simple sintered bone that was subcutaneously implanted into the contralateral back of BALB/c nude mice served as control group. @@@@RESULTS AND CONCLUSION:After calcinations, sintered cancel ous bone block colored chalks and the surface displayed a honeycomb porous structure, maintaining the porous structure of the natural cancel ous bone. Bone trabecular structure was of integrity, and pores were mutual y interconnected. After bone marrow stromal stem cel s were inoculated to the calcined bone, there were a lot of cel s adherent to the scaffold within 24 hours, and a large amount of extracel ular matrix at 7 days. The boundary between cel s and extracel ular matrix was unclear, and the cel s could grow and proliferate wel on the scaffold, suggesting that the cel viability was not influenced by the scaffold. Four weeks after implantation, a few of fragments were visible at the edge of sintered bone in the two groups. Bone cel s could be seen around the pores of sintered bone in the combination group, while fibrous connective tissue enveloped the sintered bone in the control group. Eight weeks later, the sintered bone partial y degraded into osteoid sheets surrounded by fibroblasts that arranged closely and was diverse in shape. In the combination group, osteoblasts arranged in line on the surface of sintered bone and infiltrated lymphocytes were scattered among the pores. In the control group, a great amount of connective tissues grew into the pores, but there was no osteogenesis. These findings indicate that the sintered cancel ous bone block pose better biocompatibility and biological safety, which can be a better scaffold for bone marrow stromal stem cel s. The composite of bone marrow stromal stem cel s and sintered bone has a good ability to induce new bone formation and the sintered bone can be used as scaffold materials for tissue engineering repair of bone defects.