CAJ | 학술논문

生物材料%纳米生物材料%神经生长因子%聚乙二醇-聚乳酸聚乙醇酸共聚物%纳米药物%复乳化溶剂扩散法%纳米粒%PC12细胞%省级基金生物材料%納米生物材料%神經生長因子%聚乙二醇-聚乳痠聚乙醇痠共聚物%納米藥物%複乳化溶劑擴散法%納米粒%PC12細胞%省級基金
생물재료%납미생물재료%신경생장인자%취을이순-취유산취을순산공취물%납미약물%복유화용제확산법%납미립%PC12세포%성급기금
biomaterials%nanobiomaterials%nerve growth factor%polyethylene glycol-polylactic acid glycolic acid copolymer%nano-drugs%complex emulsion solvent diffusion method%nanoparticles%PC12 cells%provincial grants-supported paper

背景:研究发现经表面修饰过的聚合物纳米粒能通过血脑屏障,可以改善药物对中枢神经系统疾病的疗效.目的:以生物可降解材料聚乙二醇-聚乳酸聚乙醇酸共聚物制备高包封率的载神经生长因子的纳米粒,并初步探讨其对PC12细胞的体外诱导效果评价. 方法:采用复乳化溶剂扩散法制备载牛血清白蛋白的聚乙二醇-聚乳酸聚乙醇酸共聚物纳米粒,用单因素分析及正交设计对工艺进行优化筛选;扫描电镜观察纳米粒形态;纳米粒分析仪测定平均粒径和分散指数;BCA法测定纳米粒包封率及载药量,并进一步研究纳米粒体外释放特性.得到最好的制备方案后,制备载神经生长因子的聚合物纳米粒,并以此处理PC12细胞,倒置荧光显微镜观察载神经生长因子的聚乙二醇-聚乳酸聚乙醇酸共聚物纳米粒对 PC12细胞的体外诱导状况,对其诱导效果、毒性及缓释效果进行评价. 结果与结论:最优处方制备的载牛血清蛋白的聚乙二醇-聚乳酸聚乙醇酸共聚物纳米粒呈球形、大小均匀,平均粒径(258.9±5.73) nm,包封率为(80.56±2.23)%,内水相中投药量10 mg 时载药量约(4.24±0.12)%,体外释放符合Higuchi方程,分初期突释释放和后期缓释释放2个阶段,0-56 d的累积释放总量分别为76.61%(牛血清白蛋白)、62.34%(神经生长因子).载神经生长因子的聚乙二醇-聚乳酸聚乙醇酸共聚物纳米粒可以诱导PC12细胞像神经元样分化效能,表现出良好的缓释性能及无毒性作用.提示制备的载神经生长因子的聚乙二醇-聚乳酸聚乙醇酸共聚物纳米粒理化性质优良,在体外有良好的缓释效能及无毒性作用.
배경:연구발현경표면수식과적취합물납미립능통과혈뇌병장,가이개선약물대중추신경계통질병적료효.목적:이생물가강해재료취을이순-취유산취을순산공취물제비고포봉솔적재신경생장인자적납미립,병초보탐토기대PC12세포적체외유도효과평개. 방법:채용복유화용제확산법제비재우혈청백단백적취을이순-취유산취을순산공취물납미립,용단인소분석급정교설계대공예진행우화사선;소묘전경관찰납미립형태;납미립분석의측정평균립경화분산지수;BCA법측정납미립포봉솔급재약량,병진일보연구납미립체외석방특성.득도최호적제비방안후,제비재신경생장인자적취합물납미립,병이차처리PC12세포,도치형광현미경관찰재신경생장인자적취을이순-취유산취을순산공취물납미립대 PC12세포적체외유도상황,대기유도효과、독성급완석효과진행평개. 결과여결론:최우처방제비적재우혈청단백적취을이순-취유산취을순산공취물납미립정구형、대소균균,평균립경(258.9±5.73) nm,포봉솔위(80.56±2.23)%,내수상중투약량10 mg 시재약량약(4.24±0.12)%,체외석방부합Higuchi방정,분초기돌석석방화후기완석석방2개계단,0-56 d적루적석방총량분별위76.61%(우혈청백단백)、62.34%(신경생장인자).재신경생장인자적취을이순-취유산취을순산공취물납미립가이유도PC12세포상신경원양분화효능,표현출량호적완석성능급무독성작용.제시제비적재신경생장인자적취을이순-취유산취을순산공취물납미립이화성질우량,재체외유량호적완석효능급무독성작용.
@@@@BACKGROUND:It is found that the surface-modified polymer nanoparticles can pass through the blood-brain barrier, and improve the drug effects on the central nervous system diseases. @@@@OBJECTIVE:To prepare nerve growth factor-loaded high encapsulation efficiency nanoparticles with a iodegradable material, polyethylene glycol-polylactic acid glycolic acid copolymer, and to explore its effect on PC12 cel s. @@@@METHODS:Bovine serum albumin-loaded polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles were prepared using complex emulsion solvent diffusion method. The production process was screened by univariate analysis and orthogonal design method. The nanoparticle morphology was observed under scanning electron microscopy, and the average particle diameter and the dispersion index were measured by nanoparticle analyzer. The nanoparticle encapsulation efficiency and drug loading were analyzed by BCA method. Nanoparticle release in vitro was also investigated. After optimization of the preparation program, nerve growth factor-loaded polymer nanoparticles were prepared and applied to PC12 cel s. The cel s were then observed by inverted fluorescence microscope, and the induction effects, toxicity and slow-release effect of polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles loaded with nerve growth factor were evaluated. @@@@RESULTS AND CONCLUSION:Bovine serum albumin-loaded polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles were prepared by optimal method. The nanoparticles were spherical, and uniform in size, with an average particle size of (258.9±5.73) nm, and the encapsulation efficiency was (80.56±2.23)%. When the dosage within the aqueous phase was 10 mg, the drug loading was (4.24±0.12)%. The in vitro release of nanoparticles was in accordance with the Higuchi equation of. Release of the nanoparticles could be divided into two stages:initial burst release stage and late sustained release. The total cumulative release amount of bovine serum albumin and nerve growth factor in 0-56 days was 76.61%and 62.34%, respectively. The PC12 cel s could be induced to differentiation by polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles loaded with nerve growth factor, and the nanoparticles exhibited good release properties and no toxic effects. It was showed that nerve growth factor-loaded polyethylene glycol-polylactic acid glycolic acid copolymer nanoparticles prepared by the optimal method had excel ent physicochemical properties, good release properties in vitro and no toxic effects.