CAJ | 학술논문

背景:异体肌腱移植是目前修复肌腱缺损的理想方法,但移植后的排斥反应是其使用受到限制的主要原因. 目的:观察脱细胞处理的版纳近交系微型猪肌腱移植修复兔跟腱缺损的疗效,以及其作为异种肌腱移植支架材料的可行性. 方法:将40只日本大白兔制作双后肢跟腱缺损实验动物模型后,随机均分为2组,脱细胞猪肌腱组用脱细胞版纳近交系微型猪肌腱修复,自体肌腱组用自体肌腱修复,术后用3-0肌腱线改良HEMI-KESSLER法进行端端原位吻合. 结果与结论:①移植后2周内,脱细胞猪肌腱组与自体肌腱组白细胞计数、C-反应蛋白测量结果差异无显著性意义(P >0.05).②两组移植后局部反应小,伤口一期愈合,屈踝功能恢复正常.③组织学检查均未见明显淋巴细胞浸润,肌腱缝合处胶原纤维相互衔接.结果说明脱细胞版纳近交系微型猪肌腱能成功修复兔跟腱缺损,且具有组织相容性好、移植排斥反应轻的优点.
배경:이체기건이식시목전수복기건결손적이상방법,단이식후적배척반응시기사용수도한제적주요원인. 목적:관찰탈세포처리적판납근교계미형저기건이식수복토근건결손적료효,이급기작위이충기건이식지가재료적가행성. 방법:장40지일본대백토제작쌍후지근건결손실험동물모형후,수궤균분위2조,탈세포저기건조용탈세포판납근교계미형저기건수복,자체기건조용자체기건수복,술후용3-0기건선개량HEMI-KESSLER법진행단단원위문합. 결과여결론:①이식후2주내,탈세포저기건조여자체기건조백세포계수、C-반응단백측량결과차이무현저성의의(P >0.05).②량조이식후국부반응소,상구일기유합,굴과공능회복정상.③조직학검사균미견명현림파세포침윤,기건봉합처효원섬유상호함접.결과설명탈세포판납근교계미형저기건능성공수복토근건결손,차구유조직상용성호、이식배척반응경적우점.
@@@@BACKGROUND:Al ogeneic tendon graft is an ideal method to repair tendon defects. But post-transplantation rejections limit the use of al ogeneic tendons. @@@@OBJECTIVE:To investigate the effect of acel ular tendon of Banna mini-pig inbred-lines on repair of rabbit Achiles tendon, and then to evaluate the feasibility and validity of porcine-derived xenogeneic graft. @@@@METHODS:Forty Japanese white rabbits were enrol ed to prepare Achil es tendon defect models of the both hind legs, and then were randomly divided into two groups:acel ular porcine tendon group treated with acel ular tendon from Banna mini-pig inbred-lines and autologous tendon group treated with rabbit autologous tendon. End-to-end anastomosis was performed with a 3-0 tendon thread using improved HEMI-KESSLER method. @@@@RESULTS AND CONCLUSION:Within 2 weeks post transplantation, there were no significant differences in white blood cel count and C-reactive protein level between the two groups (P>0.05). Local reactions in the two groups after transplantation were few, the wound healed at stage Ⅰ, and flexor ankle function returned to normal. Histological results exhibited no lymphocytic infiltration, but interconnected col agen fibers at tendon suture site. These findings indicate that acel ular tendons from Banna mini-pig inbred-lines can successful y reconstruct defected rabbit Achil es tendon with good histocompatibility and little rejection.