CAJ | 학술논문

背景:目前对富白细胞-血小板血浆凝胶的研究主要集中在生长因子的释放及其促进组织和创面生长的作用机制方面. 目的:通过测定富白细胞-血小板血浆凝胶中释放的白细胞介素1,4,6和血小板源性生长因子BB、转化生长因子β、血管内皮生长因子的水平,观察富白细胞-血小板血浆凝胶超微结构,对其抗菌作用和促进溃疡愈合的作用机制进行进一步探讨. 方法:取健康志愿者空腹静脉血离心、分离得到富白细胞-血小板血浆,再以10∶1的比例加入激活剂后混匀得富白细胞-血小板血浆凝胶.将凝胶保存在DMEM培养基中,分别于培养1,3,7,14,21 d后收集含有渗出液的培养基进行实验. 结果与结论:酶联免疫吸附法检测结果显示,健康志愿者富白细胞-血小板血浆凝胶中各类白细胞所占比例与全血有所不同,淋巴细胞所占比例最大.白细胞介素1在培养0-1 d达到峰值(P <0.05),之后逐渐下降.白细胞介素4各时间段释放量无差异.白细胞介素6的释放量在培养0-1 d,2-3 d和4-7 d保持较高水平,培养8-14 d和15-21 d释放量明显下降(P<0.05).血小板源性生长因子BB和转化生长因子β在培养1 d释放量最大(P<0.05),之后迅速下降.血管内皮生长因子表现为培养7 d逐渐上升,培养8-21 d缓慢下降.扫描电镜观察可见,凝胶中绝大部分白细胞为淋巴细胞.结果证实,富白细胞-血小板血浆凝胶的促进创面愈合的机制除与血小板源性生长因子 BB、转化生长因子β、血管内皮生长因子的释放有关外,可能与富集更多的淋巴细胞及白细胞介素1,4,6的释放有关.
배경:목전대부백세포-혈소판혈장응효적연구주요집중재생장인자적석방급기촉진조직화창면생장적작용궤제방면. 목적:통과측정부백세포-혈소판혈장응효중석방적백세포개소1,4,6화혈소판원성생장인자BB、전화생장인자β、혈관내피생장인자적수평,관찰부백세포-혈소판혈장응효초미결구,대기항균작용화촉진궤양유합적작용궤제진행진일보탐토. 방법:취건강지원자공복정맥혈리심、분리득도부백세포-혈소판혈장,재이10∶1적비례가입격활제후혼균득부백세포-혈소판혈장응효.장응효보존재DMEM배양기중,분별우배양1,3,7,14,21 d후수집함유삼출액적배양기진행실험. 결과여결론:매련면역흡부법검측결과현시,건강지원자부백세포-혈소판혈장응효중각류백세포소점비례여전혈유소불동,림파세포소점비례최대.백세포개소1재배양0-1 d체도봉치(P <0.05),지후축점하강.백세포개소4각시간단석방량무차이.백세포개소6적석방량재배양0-1 d,2-3 d화4-7 d보지교고수평,배양8-14 d화15-21 d석방량명현하강(P<0.05).혈소판원성생장인자BB화전화생장인자β재배양1 d석방량최대(P<0.05),지후신속하강.혈관내피생장인자표현위배양7 d축점상승,배양8-21 d완만하강.소묘전경관찰가견,응효중절대부분백세포위림파세포.결과증실,부백세포-혈소판혈장응효적촉진창면유합적궤제제여혈소판원성생장인자 BB、전화생장인자β、혈관내피생장인자적석방유관외,가능여부집경다적림파세포급백세포개소1,4,6적석방유관.
@@@@BACKGROUND: Current studies about leukocyte- and platelet-rich plasma gel mainly focus on growth factor release and the mechanism underlying promoting tissue growth and wound healing. @@@@OBJECTIVE:To investigate the mechanism of leukocyte-and platelet-rich plasma gel by measuring the levels of interleukin-1, interleukin-4, interleukin-6, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor released from leukocyte- and platelet-rich plasma gel and observing the ultrastructure of leukocyte-and platelet-rich plasma gel. @@@@METHODS:Blood samples were col ected from 12 healthy volunteers. Leukocyte-and platelet-rich plasma was produced by two-step centrifugation, and leukocyte-and platelet-rich plasma gel was obtained by combining the leukocyte-and platelet-rich plasma with thrombin and calcium gluconate (10:1). The leukocyte-and platelet-rich plasma gel was preserved in Dulbecco’s modified Eagle’s medium, and the Dulbecco’s modified Eagle’s medium including exudates of leukocyte-and platelet-rich plasma gel was col ected at days 1, 3, 7, 14, 21. @@@@RESULTS AND CONCLUSION:Enzyme linked immunoabsorbent assay showed that the leukocyte formula in the leukocyte-and platelet-rich plasma gel was different from that in the whole blood, and lymphocytes shared the largest proportion. Interleukin-1 level peaked at 0-1 day of culture (P<0.05), and then gradual y decreased. Interleukin-4 level showed no changes at different periods. Interleukin-6 maintained a higher release at 0-1, 2-3, and 4-7 days, but dramatical y reduced at 8-14 and 15-21 days of culture (P<0.05). The release amount of platelet-derived growth factor-BB, transforming growth factor-β1 reached the peak at 1 day of culture (P<0.05), and then exhibited a raped decline. The release amount of vascular endothelial growth factor gradual y increased at 7 days of culture, and then gradual y declined at 8-21 days of culture. Under the scanning electron microscope, vast majority of leukocytes in the leukocyte-and platelet-rich plasma gel were confirmed as lymphocytes. Results showed that besides the expression of growth factors such as platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor, the concentrations and components change of lymphocytes and expression of inflammatory factors such as interleukin-1, interleukin-4, and interleukin-6 might be one of the mechanisms by which leukocyte-and platelet-rich plasma gel can promote wound healing.