CAJ | 학술논문

背景:前期研究发现,中国人肝细胞系1细胞分化程度高且生物代谢功能良好,并且中国人肝细胞系1细胞组织学上来源于正常肝组织,较其他来源于肿瘤源性的肝细胞系更为安全. 目的:探讨中国人肝细胞系中国人肝细胞系1细胞在混合型生物人工肝中的生物代谢功能. 方法:15只食蟹猴随机分成对照组(n=5)和治疗组(n=10),均建立急性肝功能衰竭模型,治疗组接受以全接触灌流型生物反应器接种微载体微重力中国人肝细胞系1细胞建立的人源细胞混合型生物人工肝进行治疗. 结果与结论:急性肝功能衰竭食蟹猴血清谷氨酸转氨酶、总胆红素、总胆汁酸、尿素氮、肌酐、血氨均明显上升,而白蛋白、Fischer 指数则显著下降;人源细胞混合型生物人工肝治疗后,急性肝功能衰竭食蟹猴血清谷氨酸转氨酶、总胆红素、总胆汁酸、尿素氮、肌酐、血氨和白蛋白均恢复.提示中国人肝细胞系1细胞在混合型生物人工肝中生物代谢功能良好,表现出良好的肝特异性生物合成及生物代谢功能.
배경:전기연구발현,중국인간세포계1세포분화정도고차생물대사공능량호,병차중국인간세포계1세포조직학상래원우정상간조직,교기타래원우종류원성적간세포계경위안전. 목적:탐토중국인간세포계중국인간세포계1세포재혼합형생물인공간중적생물대사공능. 방법:15지식해후수궤분성대조조(n=5)화치료조(n=10),균건립급성간공능쇠갈모형,치료조접수이전접촉관류형생물반응기접충미재체미중력중국인간세포계1세포건립적인원세포혼합형생물인공간진행치료. 결과여결론:급성간공능쇠갈식해후혈청곡안산전안매、총담홍소、총담즙산、뇨소담、기항、혈안균명현상승,이백단백、Fischer 지수칙현저하강;인원세포혼합형생물인공간치료후,급성간공능쇠갈식해후혈청곡안산전안매、총담홍소、총담즙산、뇨소담、기항、혈안화백단백균회복.제시중국인간세포계1세포재혼합형생물인공간중생물대사공능량호,표현출량호적간특이성생물합성급생물대사공능.
@@@@BACKGROUND:Our preliminary study found that Chinese human liver cel line-1 has high differentiation ability and good biological metabolic functions, and the human liver cel lines are separated from normal liver tissue on a cel histology which are safer than the tumor-derived liver cel line. @@@@OBJECTIVE:To investigate the biological metabolic functions of Chinese human liver cel line-1 cel s in a hybrid bioartificial liver. @@@@METHODS:Fifteen cynomolgus monkeys were randomly divided into the control group (n=5) and the treatment group (n=10), and al the monkeys were used to establish the acute liver failure models. The models in the treatment group were implanted with Chinese human liver cel line-1 established hybrid bioartificial liver through the contact perfusion bioreactor inoculation under micro carrier microgravity. @@@@RESULTS AND CONCLUSION:The serum alanine aminotransferase, total bilirubin, total bile acid, urea nitrogen, creatinine and blood ammonia levels of the cynomolgus monkeys with acute liver failure were increased, while the albumin level and Fischer index were decreased;after treated with hybrid bioartificial liver, serum alanine aminotransferase, total bilirubin, total bile acid, urea nitrogen, creatinine and blood ammonia levels of the cynomolgus monkeys with acute liver failure were recovered to the normal levels. It suggests that Chinese human liver cel line-1 cel s in hybrid bioartificial liver have good biological metabolic function with good liver-specific biosynthesis and biological metabolic functions.