CAJ | 학술논문

器官移植%组织移植%脂肪移植%脂肪细胞%胰岛素%微血管%血管内皮细胞%血管吻合%存活率%纤维组织%纤维化%糖代谢%省级基金器官移植%組織移植%脂肪移植%脂肪細胞%胰島素%微血管%血管內皮細胞%血管吻閤%存活率%纖維組織%纖維化%糖代謝%省級基金
기관이식%조직이식%지방이식%지방세포%이도소%미혈관%혈관내피세포%혈관문합%존활솔%섬유조직%섬유화%당대사%성급기금
organ transplantation%tissue transplantation%fat transplantation%adipocytes%insulin%microvessels%vascular endothelial cells%vascular anastomosis%survival rate%fibrous tissue%fibrosis%glucose metabolism%provincial grants-supported paper

背景:细胞培养实验发现胰岛素不但能促进血管内皮细胞的增殖和修复,还能有效促进前脂肪细胞的增殖和分化,体内实验观察胰岛素对移植脂肪新生微血管的影响罕有报道. 目的:探讨胰岛素对大鼠移植脂肪内微血管形成的作用. 方法:将48只SD大鼠随机分成胰岛素组和对照组,从大鼠输卵管旁取出1 mL脂肪组织,制成脂肪颗粒移植到大鼠背部,建立颗粒脂肪移植模型.胰岛素组在移植前将脂肪颗粒放入浓度为300 mU/L的胰岛素中预处理;对照组对移植脂肪组织不施加处理因素.在移植后10,20,28 d取移植的脂肪组织做苏木精-伊红和CD34血管染色,观测移植脂肪细胞变化和微血管生长情况. 结果与结论:苏木精-伊红染色切片中可见移植体内有成熟脂肪细胞,细胞体积变小,部分脂肪细胞有破裂萎缩现象,细胞间有纤维组织增生,胰岛素组脂肪细胞周围纤维组织少于对照组.在10,20 d,胰岛素组微血管密度为(3.92±0.12)个/HP和(6.96±0.42)个/HP,对照组为(2.05±0.21)个/HP和(4.40±0.36)个/HP,胰岛素组微血管密度均高于对照组,两组比较差异有显著性意义(P<0.01).提示胰岛素能促进移植脂肪块内微血管生长,提高移植脂肪的存活率.
배경:세포배양실험발현이도소불단능촉진혈관내피세포적증식화수복,환능유효촉진전지방세포적증식화분화,체내실험관찰이도소대이식지방신생미혈관적영향한유보도. 목적:탐토이도소대대서이식지방내미혈관형성적작용. 방법:장48지SD대서수궤분성이도소조화대조조,종대서수란관방취출1 mL지방조직,제성지방과립이식도대서배부,건립과립지방이식모형.이도소조재이식전장지방과립방입농도위300 mU/L적이도소중예처리;대조조대이식지방조직불시가처리인소.재이식후10,20,28 d취이식적지방조직주소목정-이홍화CD34혈관염색,관측이식지방세포변화화미혈관생장정황. 결과여결론:소목정-이홍염색절편중가견이식체내유성숙지방세포,세포체적변소,부분지방세포유파렬위축현상,세포간유섬유조직증생,이도소조지방세포주위섬유조직소우대조조.재10,20 d,이도소조미혈관밀도위(3.92±0.12)개/HP화(6.96±0.42)개/HP,대조조위(2.05±0.21)개/HP화(4.40±0.36)개/HP,이도소조미혈관밀도균고우대조조,량조비교차이유현저성의의(P<0.01).제시이도소능촉진이식지방괴내미혈관생장,제고이식지방적존활솔.
@@@@BACKGROUND:Currently, the in vitro cel culture experiment shows that insulin can not only promote the proliferation and repairing of vascular endothelia cel s, but also can increase the proliferation and differentiation of preadipocytes. However, there are a few papers about the effect of insulin on revascularization in fat grafts through in vivo experiment. @@@@OBJECTIVE:To investigate the influence of insulin on the microvessel formation in fat grafts of rats. @@@@METHODS:Forty-eight Sprague Dawley female rats were used in this study, and then the rats were randomly divided into insulin group and control group. 1 mL fat graft was obtained from the side of fal opian tubes of the rats to make fat particles and then transplanted to the dorsal of the rats to establish the granule fat graft models. In the insulin group, granule fat grafts were pre-treated with insulin with the concentration of 300 mU/L before transplantation;the fat grafts in the control group without any treatment. The samples of fat grafts were harvested at 10, 20 and 28 days after the transplantation and treated with hematoxylin-eosin staining and CD34 vascular staining respectively. The changes of grafted adipocytes and the growth of microvessel were observed. @@@@RESULTS AND CONCLUSION:Mature adipocytes could be observed in the hematoxylin-eosin staining sections, the size of adipocytes was smal er than that before transplantation, and part of the adipocytes were ruptured and shrinked. Hyperplasia of fibrous tissue was observed in two groups, and the number of fibrous tissue around the adipocytes in the insulin group was smal er than that in the control group. At 10 and 20 days after transplantation, the microvessel densities were (3.92±0.12) microvessels/high power field and (6.96±0.42) microvessels/high power field in the insulin group, (2.05±0.21) microvessels/high power field and (4.40±0.36) microvessels/high power field in the control group, respectively. Microvessel density in insulin group was higher than that in the control group, and there was a significant difference between two groups (P<0.01). The result shows that insulin can improve the survival rate of autogenous fat grafts by promoting the microvessel formation.