CAJ | 학술논문

器官移植%组织移植%器官移植临床应用%脂肪干细胞%成脂分化%背阔肌筋膜瓣%臀大肌筋膜瓣%组织工程化脂肪%脂肪组织%脂肪细胞%血管生成%细胞移植%胶原支架%兔%组织工程%省级基金器官移植%組織移植%器官移植臨床應用%脂肪榦細胞%成脂分化%揹闊肌觔膜瓣%臀大肌觔膜瓣%組織工程化脂肪%脂肪組織%脂肪細胞%血管生成%細胞移植%膠原支架%兔%組織工程%省級基金
기관이식%조직이식%기관이식림상응용%지방간세포%성지분화%배활기근막판%둔대기근막판%조직공정화지방%지방조직%지방세포%혈관생성%세포이식%효원지가%토%조직공정%성급기금

背景:再血管化是构建组织工程化脂肪的关键因素. 目的:观察3种不同筋膜瓣包裹的脂肪来源干细胞与胶原蛋白支架复合物在体内成脂效率的差异. 方法:分离兔右侧带血管蒂的背阔肌筋膜瓣,包裹成脂诱导后的脂肪来源干细胞与Ⅰ型胶原蛋白复合物,设为带有轴型血管蒂的诱导分化组.分离兔左侧带血管蒂的背阔肌筋膜瓣,包裹未经诱导的脂肪来源干细胞与Ⅰ型胶原蛋白复合物,设为带有轴型血管蒂的未诱导分化组.分离兔右侧无特定血管蒂的臀大肌筋膜瓣,包裹未经诱导的脂肪来源干细胞与Ⅰ型胶原蛋白复合物,作为对照组. 结果与结论:移植后8周,苏木精-伊红染色和免疫组织化学染色检测结果显示,各组均可见新生脂肪组织形成,带有轴型血管蒂的诱导分化组新生肪组织平均湿质量和新生微血管密度均高于其他2组(P<0.01,P<0.05).结果说明实验成功构建了带血管肌筋膜包埋成脂诱导后的脂肪干细胞载体复合物构建血管化组织工程脂肪,此复合物在体内的成脂效率和促进血管再生的能力最好.
배경:재혈관화시구건조직공정화지방적관건인소. 목적:관찰3충불동근막판포과적지방래원간세포여효원단백지가복합물재체내성지효솔적차이. 방법:분리토우측대혈관체적배활기근막판,포과성지유도후적지방래원간세포여Ⅰ형효원단백복합물,설위대유축형혈관체적유도분화조.분리토좌측대혈관체적배활기근막판,포과미경유도적지방래원간세포여Ⅰ형효원단백복합물,설위대유축형혈관체적미유도분화조.분리토우측무특정혈관체적둔대기근막판,포과미경유도적지방래원간세포여Ⅰ형효원단백복합물,작위대조조. 결과여결론:이식후8주,소목정-이홍염색화면역조직화학염색검측결과현시,각조균가견신생지방조직형성,대유축형혈관체적유도분화조신생방조직평균습질량화신생미혈관밀도균고우기타2조(P<0.01,P<0.05).결과설명실험성공구건료대혈관기근막포매성지유도후적지방간세포재체복합물구건혈관화조직공정지방,차복합물재체내적성지효솔화촉진혈관재생적능력최호.
@@@@BACKGROUND:Revascularization mechanism is the decisive factor for the successful construction of tissue-engineered adipose tissue. @@@@OBJECTIVE:To observe the difference of in vivo adipogenic efficacy of adipose-derived stem cel s and col agen protein scaffold encapsulated in three muscular fasciae. @@@@METHODS:The rabbit right vascularized latissimus dorsi muscular fasciae was separated and encapsulated with type Ⅰ col agen protein and adipogenic differentiated adipose-derived stem cel s complexes as the differentiation group with axial pattern blood vessel pedicle. The rabbit left vascularized latissimus dorsi muscular fasciae was separated and encapsulated with type Ⅰ col agen protein and undifferentiated adipose-derived stem cel s complexes as the undifferentiation group with axial pattern blood vessel pedicle. The rabbit gluteus maximus muscular fasciae without specific vascular pedicle was separated and encapsulated with type Ⅰ col agen protein and undifferentiated adipose-derived stem cel s complexes as the control group. @@@@RESULTS AND CONCLUSION:Eight weeks after transplantation, hematoxylin-eosin staining and immunohistochemical staining showed that there was new adipose tissue formation in al groups. The mean humid weight of the neonatal fat tissue and the microvessel density in the differentiation group was higher than those in the undifferentiation group and the control group (P<0.01, P<0.05). It indicated that the vascularized tissue-engineered adipose was successful y established via adipose-derived stem cel s-attached scaffolds encapsulated in muscular fasciae with axial pattern blood vessel pedicle with good in vivo adipogenic efficiency and strong ability to promote angiogenesis.