中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
18期
156-163
,共8页
器官移植%器官移植综述%深低温保存%复合组织%玻璃化%保护剂%复温%降温%断肢再植%异体移植%渗透性损伤%研究进展
器官移植%器官移植綜述%深低溫保存%複閤組織%玻璃化%保護劑%複溫%降溫%斷肢再植%異體移植%滲透性損傷%研究進展
기관이식%기관이식종술%심저온보존%복합조직%파리화%보호제%복온%강온%단지재식%이체이식%삼투성손상%연구진전
organ transplantation%organ transplantation review%cryopreservation%composite tissue%vitrification%cryoprotectant agent%rewarming%cooling%limb replantation%al ograft%permeability injury%research progress
背景:深低温冻存可以使离体组织的活性及功能最大限度的保留,对医学研究、临床治疗起到巨大的推动作用.但在复合组织深低温冻存中,保护剂及应用还存在着很多问题有待解决.
目的:综述近年国内外深低温冻存中保护剂应用的研究进展.
方法:第一作者应用计算机检索2003年1月至2012年12月PubMed数据库、中国期刊全文数据库、万方数据库相关文章,英文检索词为“cryopreservation,cryoprotectant,composite tissues,vitrification, transplantation”;中文检索词为“深低温保存,保护剂,复合组织,玻璃化,移植”.共检索到143篇相关文献,67篇文献符合纳入标准.
结果与结论:自上世纪开始国内外许多学者经过大量动物细胞和组织的基础实验研究,不断探寻各种细胞组织的冻存方法,采用了多种多样的冻存技术,使用的保护剂种类繁多,取得一定的研究成果,发现保护剂的应用在深低温组织冻存中起到了至关重要的作用,深低温冻存在组织工程、生殖医学、移植等领域已有广泛的应用.但目前复合组织深低温冻存保护剂的应用尚处于研究阶段,保护剂的种类、浓度以及导入洗脱方法均直接影响最终组织保存的成败.
揹景:深低溫凍存可以使離體組織的活性及功能最大限度的保留,對醫學研究、臨床治療起到巨大的推動作用.但在複閤組織深低溫凍存中,保護劑及應用還存在著很多問題有待解決.
目的:綜述近年國內外深低溫凍存中保護劑應用的研究進展.
方法:第一作者應用計算機檢索2003年1月至2012年12月PubMed數據庫、中國期刊全文數據庫、萬方數據庫相關文章,英文檢索詞為“cryopreservation,cryoprotectant,composite tissues,vitrification, transplantation”;中文檢索詞為“深低溫保存,保護劑,複閤組織,玻璃化,移植”.共檢索到143篇相關文獻,67篇文獻符閤納入標準.
結果與結論:自上世紀開始國內外許多學者經過大量動物細胞和組織的基礎實驗研究,不斷探尋各種細胞組織的凍存方法,採用瞭多種多樣的凍存技術,使用的保護劑種類繁多,取得一定的研究成果,髮現保護劑的應用在深低溫組織凍存中起到瞭至關重要的作用,深低溫凍存在組織工程、生殖醫學、移植等領域已有廣汎的應用.但目前複閤組織深低溫凍存保護劑的應用尚處于研究階段,保護劑的種類、濃度以及導入洗脫方法均直接影響最終組織保存的成敗.
배경:심저온동존가이사리체조직적활성급공능최대한도적보류,대의학연구、림상치료기도거대적추동작용.단재복합조직심저온동존중,보호제급응용환존재착흔다문제유대해결.
목적:종술근년국내외심저온동존중보호제응용적연구진전.
방법:제일작자응용계산궤검색2003년1월지2012년12월PubMed수거고、중국기간전문수거고、만방수거고상관문장,영문검색사위“cryopreservation,cryoprotectant,composite tissues,vitrification, transplantation”;중문검색사위“심저온보존,보호제,복합조직,파리화,이식”.공검색도143편상관문헌,67편문헌부합납입표준.
결과여결론:자상세기개시국내외허다학자경과대량동물세포화조직적기출실험연구,불단탐심각충세포조직적동존방법,채용료다충다양적동존기술,사용적보호제충류번다,취득일정적연구성과,발현보호제적응용재심저온조직동존중기도료지관중요적작용,심저온동존재조직공정、생식의학、이식등영역이유엄범적응용.단목전복합조직심저온동존보호제적응용상처우연구계단,보호제적충류、농도이급도입세탈방법균직접영향최종조직보존적성패.
@@@@BACKGROUND:Cryopreservation can preserve the activity and function of the in vitro tissues in the greatest degree, which create the enormous push and facilitation effect in medical research and clinical treatment. But in the composite tissue cryopreservation, there also has many problems in the application of cryoprotectant waiting for solve. @@@@OBJECTIVE:To review the domestic and foreign research progress in the application of cryoprotectant in tissue cryopreservation @@@@METHODS:The PubMed database, Chinese journal ful-text database and Wanfang database were searched by the first author for the literatures published from January 2003 to December 2012. The key words were“cryopreservation, cryoprotectant, composite tissues, vitrification, transplantation”in Chinese and English. Total y 143 relevant literatures were col ected, and 56 of them were in accordance with the inclusion criteria. @@@@RESULTS AND CONCLUSION:Since the beginning of the last century, domestic and overseas experts have made great efforts on the research of tissue cryopreservation through a lot of fundamental experimental researches of animal cel s and tissues, and variety of cryopreservation techniques and various cryoprotectants were applied and obtained a certain achievements, it found that the application of cryoprotectant played a crucial role in tissue cryopreservation. Cryopreservation is widely applied in some domains, such as tissue engineering, reproductive medicine and transplantation field. But at present, the application of cryoprotectant in composite tissue cryopreservation is stil under studying in laboratory. The types and concentrations of cryoprotectant, as wel as the addition and elution process can directly determine the ultimate success of cryopreservation.
