CAJ | 학술논문

目的建立高效液相色谱法测定盐酸丁卡因麻黄碱溶液中盐酸丁卡因的含量方法.方法采用Agilent ZORBAX SB-C18色谱柱;以0.05mol/L磷酸二氢钾溶液[用稀磷酸或三乙胺调节pH值至(3.0±0.1)]-乙腈(70∶30);检测波长:310nm;流速:1mL/min;进样量:20μL;柱温:30℃.结果盐酸丁卡因在1.02~50.95μg/mL范围内线性良好(r2=1),平均回收率为100.31%,RSD为0.61%(n=9).结论该方法可有效的测定样品中盐酸丁卡因的含量,精密度高、方法简便准确可靠.
목적건립고효액상색보법측정염산정잡인마황감용액중염산정잡인적함량방법.방법채용Agilent ZORBAX SB-C18색보주;이0.05mol/L린산이경갑용액[용희린산혹삼을알조절pH치지(3.0±0.1)]-을정(70∶30);검측파장:310nm;류속:1mL/min;진양량:20μL;주온:30℃.결과염산정잡인재1.02~50.95μg/mL범위내선성량호(r2=1),평균회수솔위100.31%,RSD위0.61%(n=9).결론해방법가유효적측정양품중염산정잡인적함량,정밀도고、방법간편준학가고.
@@@@Objective To establish a HPLC method for determining the content of tetracaine hydrochloride in tetracaine hydrochloride and ephedrine hydrochloride solution. Methods The separation was performed on agilent ZORBAX SB-C18. The mobile phase was composed of 0.05mol/L potassium dihydrogen phosphate solution [adjusted with phosphoric acid or triethylamine to pH(3±0.1)]-acetonitrile (70∶30). The detection wavelength was 310nm, the flow rate was 1.0mL/min, and the column temperature was 30℃. Results The linear range of tetracaine hydrochloride was 1.02-50.95μg/mL(r 2=1), the average recovery was 100.31%, and the RSD was 0.61%(n=9). Conclusion This method can be effective determination of the content of tetracaine hydrochloride, high precision, the method is simple, accurate and reliable.