CAJ | 학술논문

目的小鼠提取的树突状细胞(DC)和小鼠结肠癌细胞CT26.WT共培养、融合,CD44v6基因来修饰融合细胞,研究CD44v6修饰DC融合瘤苗体外抗结肠癌的作用机制,为肿瘤预防及免疫治疗提供实验依据.方法①在聚乙二醇的作用下,DC细胞和CT26.WT细胞共培养、融合,利用HAT/HT筛选系统筛选出纯净DC融合细胞.在脂质体的作用下,将pBud-CD44v6转染到DC融合细胞中表达;②体外实验观察DC融合瘤苗对小鼠结肠癌细胞的杀灭作用.结果构建了基因CD44v6修饰的DC/CT26.WT肿瘤融合疫苗;实验组细胞的淋巴细胞毒(CTL)活性明显高于对照组.结论 CD44v6修饰的DC融合瘤苗可有效诱导T淋巴细胞产生强大抗结肠癌作用.
목적소서제취적수돌상세포(DC)화소서결장암세포CT26.WT공배양、융합,CD44v6기인래수식융합세포,연구CD44v6수식DC융합류묘체외항결장암적작용궤제,위종류예방급면역치료제공실험의거.방법①재취을이순적작용하,DC세포화CT26.WT세포공배양、융합,이용HAT/HT사선계통사선출순정DC융합세포.재지질체적작용하,장pBud-CD44v6전염도DC융합세포중표체;②체외실험관찰DC융합류묘대소서결장암세포적살멸작용.결과구건료기인CD44v6수식적DC/CT26.WT종류융합역묘;실험조세포적림파세포독(CTL)활성명현고우대조조.결론 CD44v6수식적DC융합류묘가유효유도T림파세포산생강대항결장암작용.
@@@@Objective To survey the antitumor mechanism and effect of fusion hybrid cells of DCs and colon cancer cell CT26.WT co-cultivating and expressing CD44v6 gene, DCs and colon cancer cell CT26.WT derived from mice were blended, and modified with CD44v6 gene, it will provide the experimental foundation on the prevention and immunotherapy for tumor patients. Methods①CT26.WT cells and DCs were blended by polyethylene glycal, and the fused cells were screened by HAT/HT system. The recombinant pBud-CD44v6 vector was transfected into the fused cells by lipofectamine, and to express;②The anticancer effect and cytotoxicty of fused–cell vaccine were studied in vitro. Result DC/CT26.WT fused cell vaccine modified with CD44v6 geng was constructed;the lymphocyte cytotoxicity T(CTL) of immune group was larger activity than that of the anainst group .Conclusion The fused cells of DC expressing CD44v6 geng can induce strong anticolon-cancer activity of T lymphocyte.