CAJ | 학술논문

阔叶十大功劳、狭叶十大功劳及南天竹同为小檗科植物,其叶片表观性状存在较大差异.为了考察上述3种植物叶片SPAD值与叶绿素的相关性,运用SPAD‐502Plus叶绿素仪和分光光度法分别测定这3种植物叶片的叶绿素含量,并引入叶鲜质量与叶面积比( ratio of leaf fresh mass and leaf area ,SLW)作为叶片厚薄的特征参数;再应用通径分析进一步探讨叶片特征参数和各叶绿素参数对SPAD值的直接或间接影响,最后建立SPAD值与叶绿素a及叶绿素b和总叶绿素的线性函数、二次多项式函数、指数函数及乘幂函数的拟合方程,并根据决定系数( R2)确定最佳拟合曲线.研究表明:SPAD值与叶绿素含量在单位叶鲜质量表示下相关性不强,但在单位叶面积表示下成极显著相关;通过通径分析发现在考察的各参数中对SPAD值影响最大的是叶绿素a含量,说明SPAD‐502Plus叶绿素仪有效地消除了叶片厚度等对测量结果的影响;在所建立的拟合方程中,SPAD值与叶绿素含量的非线性拟合效果要优于线性,并且不同种植物的最佳数学模型各有不同.上述分析说明,利用SPAD‐502Plus叶绿素仪快速、无损地评估在体植物叶片的叶绿素含量是可行的.
활협십대공로、협협십대공로급남천죽동위소벽과식물,기협편표관성상존재교대차이.위료고찰상술3충식물협편SPAD치여협록소적상관성,운용SPAD‐502Plus협록소의화분광광도법분별측정저3충식물협편적협록소함량,병인입협선질량여협면적비( ratio of leaf fresh mass and leaf area ,SLW)작위협편후박적특정삼수;재응용통경분석진일보탐토협편특정삼수화각협록소삼수대SPAD치적직접혹간접영향,최후건립SPAD치여협록소a급협록소b화총협록소적선성함수、이차다항식함수、지수함수급승멱함수적의합방정,병근거결정계수( R2)학정최가의합곡선.연구표명:SPAD치여협록소함량재단위협선질량표시하상관성불강,단재단위협면적표시하성겁현저상관;통과통경분석발현재고찰적각삼수중대SPAD치영향최대적시협록소a함량,설명SPAD‐502Plus협록소의유효지소제료협편후도등대측량결과적영향;재소건립적의합방정중,SPAD치여협록소함량적비선성의합효과요우우선성,병차불동충식물적최가수학모형각유불동.상술분석설명,이용SPAD‐502Plus협록소의쾌속、무손지평고재체식물협편적협록소함량시가행적.
@@@@Determination of chlorophyll concentration has significance for photosynthesis and stress physiology since it is the main photosynthetic pigment in higher plant leaves . Previous researches have shown high correlation between leaf chlorophyll concentration and SPAD values , obtained by SPAD‐502Plus chlorophyll meter . Path analysis also has been used for analyzing the direct effect and indirect effect among various independent variables and dependent variable . @@@@There exist large differences in leaf characteristics among three species of Berberidaceae i .e Mahonia bealei ( Fort .) Carr . , Mahonia f ortunei ( Lindl .) Fedde , and Nandina domestica . In order to study the correlation of SPAD values and chlorophyll concentration in these three species , the chlorophyll concentration was measured by SPAD‐502Plus chlorophyll meter and ultraviolet spectrophotometer . The chlorophyll concentration was expressed as per unit leaf area or per unit leaf fresh mass respectively ;a parameter SLW ( ratio of leaf fresh mass and leaf area) , was introduced to describe the characteristic of leaf thickness . The effects of both the leaf apparent characteristics and chlorophyll parameters on SPAD values were further investigated by path analysis . Moreover , the empirical relationships between the SPAD values and the concentration of chlorophyll a (Chla) , chlorophyll b (Chlb) and total chlorophyll ( TChl) were determined by a linear function , a second‐order polynomials function , an exponential function and a power function to calculate the R2 decision value based optimal fitting curve . @@@@For all three species , when chlorophyll concentration was expressed as per unit leaf area , it showed significant correlation with SPAD values . The SPAD values showed higher correlation with the concentration of Chla than Chlb in M . bealei and M . f ortunei , and the result was opposite in N . domestica . The positive correlation between parameter SLW and SPAD values was found among the three species and was significant in M . bealei and M . f ortunei , which indicated that thick leaves contained higher chlorophyll concentration . Furthermore , SLW showed higher positive correlation with carotenoid (Car) than Chla , Chlb , TChl in all three species , which could be related to the stress resistance of Car ;The path analysis led to two fold discoveries: 1) The concentration of Chla had the greatest impact on SPAD values; 2) The SPAD‐502Plus chlorophyll meter could effectively eliminate the impact factors ( e .g . , leaf thickness) . A non‐linear relationship between SPAD values and chlorophyll concentration was indicated by the empirical equations;nevertheless , the optimal equations of Chla , Chlb , and TChl in M . bealei . were exponential function , while the power function of Chla , TChl and the second‐order polynomials function of Chlb in M . f ortunei and N . domestica were observed . @@@@The correlation between SPAD values and chlorophyll concentration in three different species of Berberidaceae indicats that the SPAD values can accurately reflect the chlorophyll concentration in higher plant leaves and the SPAD‐502Plus chlorophyll meter can effectively eliminate the impact factors , so we propose that the SPAD‐502Plus chlorophyll meter can be referred as a fast and nondestructive method to evaluate the chlorophyll concentration , and SPAD calibration curves shall commonly be parameterized as non‐linear relationship .