CAJ | 학술논문

查尔酮合成酶( chalcone synthase , CHS)是黄酮类物质合成的第一关键酶.为了获得 CH S基因,我们在ACGM标记所扩增的PCR片段内设计锚定引物,并结合RACE技术首次克隆了柳杉属3种植物的 CH S基因,包括柳杉( Cryptomeria j aponica var . sinensis , CjsCH S)、短茸柳杉( C . j aponica cv . Araucarioides ,Cj aCH S)和日本柳杉( C . j aponica ,CjCH S),并对其进行分析.结果表明:3个基因长都为1176 bp ,编码391个氨基酸,它们都含有CHS高度保守活性位点以及CHS标签序列GFGPG ,其编码蛋白的相似度达99%,与其他植物相似度在79%以上,表明 CH S基因在进化上具有相对保守性.
사이동합성매( chalcone synthase , CHS)시황동류물질합성적제일관건매.위료획득 CH S기인,아문재ACGM표기소확증적PCR편단내설계묘정인물,병결합RACE기술수차극륭료류삼속3충식물적 CH S기인,포괄류삼( Cryptomeria j aponica var . sinensis , CjsCH S)、단용류삼( C . j aponica cv . Araucarioides ,Cj aCH S)화일본류삼( C . j aponica ,CjCH S),병대기진행분석.결과표명:3개기인장도위1176 bp ,편마391개안기산,타문도함유CHS고도보수활성위점이급CHS표첨서렬GFGPG ,기편마단백적상사도체99%,여기타식물상사도재79%이상,표명 CH S기인재진화상구유상대보수성.
@@@@Chalcone synthase (CHS) is the first enzyme in the flavonoid biosynthetic pathway in plants . In order to clone CHS gene in Cryptomeria plants , the amplified consensus genetic markers ( ACGM ) were designed and combined with RACE technique , three CHS genes in Cryptomeria japonica var . sinensis ,C . japonica cv . Araucarioides and C . japonica were obtained , respectively . Sequence analysis showed that the coding sequence (CDS) of each CHS gene was 1 176 bp in length and encoded 391 amino acids . The GFGPG tag of CHS conservative active sites were found in their proteins . Three CHS proteins showed 99% homology with each other , but more than 79% homology with other plants , which indicated that CHS were relatively conserved during evolution .