CAJ | 학술논문

研究黄樱椒提取物对营养型肥胖小鼠的脂肪代谢干预作用.将32只4周大小的C57BL/6J小鼠分为4组:空白对照组、肥胖模型组、黄樱椒提取物高剂量组及低剂量组.除空白对照组给予正常饲料以外,其余各组均喂养高脂饲料,每天灌胃给予黄樱椒提取物1次,持续灌胃40 d ,每5 d称量1次体质量.实验结束,分别测定小鼠体质量、脂体比、Lee指数、血清三酰甘油( triglyceride , TG)、总胆固醇( total cholesterol , TC)、高密度脂蛋白( high‐density lipoprotein , HDL)、低密度脂蛋白( low‐density lipoprotein , LDL)和瘦素( leptin)含量,肝中TC、TG以及谷丙转氨酶( alanine aminotransferase , ALT )、谷草转氨酶( aspartate aminotransferase , AST )含量,以及胰脂肪酶( pancrelipase , PL)含量.与肥胖模型组比较,黄樱椒提取物能显著降低小鼠体质量( P<0.01)和脂肪总质量( P <0.01),降低其Lee指数( P<0.05);血清中TC含量降低( P<0.05),TG含量降低( P <0.05),LDL含量降低( P<0.05).不影响HDL含量,肝质量下降( P<0.01),TG含量下降( P<0.05),对TC无影响;胰脂肪酶活性降低( P <0.05).黄樱椒提取物对营养型肥胖小鼠具有一定的脂肪代谢干预作用,没有造成肝毒性,有进一步开发的意义.
연구황앵초제취물대영양형비반소서적지방대사간예작용.장32지4주대소적C57BL/6J소서분위4조:공백대조조、비반모형조、황앵초제취물고제량조급저제량조.제공백대조조급여정상사료이외,기여각조균위양고지사료,매천관위급여황앵초제취물1차,지속관위40 d ,매5 d칭량1차체질량.실험결속,분별측정소서체질량、지체비、Lee지수、혈청삼선감유( triglyceride , TG)、총담고순( total cholesterol , TC)、고밀도지단백( high‐density lipoprotein , HDL)、저밀도지단백( low‐density lipoprotein , LDL)화수소( leptin)함량,간중TC、TG이급곡병전안매( alanine aminotransferase , ALT )、곡초전안매( aspartate aminotransferase , AST )함량,이급이지방매( pancrelipase , PL)함량.여비반모형조비교,황앵초제취물능현저강저소서체질량( P<0.01)화지방총질량( P <0.01),강저기Lee지수( P<0.05);혈청중TC함량강저( P<0.05),TG함량강저( P <0.05),LDL함량강저( P<0.05).불영향HDL함량,간질량하강( P<0.01),TG함량하강( P<0.05),대TC무영향;이지방매활성강저( P <0.05).황앵초제취물대영양형비반소서구유일정적지방대사간예작용,몰유조성간독성,유진일보개발적의의.
Presently , obesity is not only becoming a critical medical problem worldwide but also will cause lots of obesity‐related chronic diseases as well , like hypertension , cardiovascular diseases , diabetes and so on . It becomes more and more important for current scientists to explore the effective pathway to solve the puzzle . Many researchers from different countries put much more emphasis on the anti‐obesity effect of natural products . There are many studies showing that the extracts of certain parts of varied kinds of natural plants are able to inhibit the obesity through the influences on lipids metabolism , adipocytes secretion and show prevention effects on obesity through animal tests . Yellow capsicum includes functional component capsaicin which has multifunctional pharmaceutical effects . In the present study , the anti‐obesity action of yellow capsicum extract ( YCE ) was investigated through their intervention effect on lipids metabolism in C57BL/6J mice . @@@@The total thirty‐two mice were divided into four groups , including control group , obesity model group and YCE treatment groups at doses of 100 and 25 mg/kg body mass . The mice in control group were fed with normal diet and those in other groups were fed with hyperlipidic diet and YCE at different doses once a day for 40 consecutive days . All mice were weighed every five days and their body mass were recorded . In the end , the mice were killed and dissected , body fat percentage , contents of total cholesterol ( TC ) , triglyceride ( TG ) , high‐density lipoprotein ( HDL ) , low‐density lipoprotein ( LDL ) and leptin in serum , expression levels of TC , TG , aspartate aminotransferase ( AST) and alanine aminotransferase ( ALT) in the liver , were all detected and analyzed . Besides , the pancrelipase ( PL ) level of pancreatic tissue was also detected through ELISA method , as it is an important lipase in lipids metabolism . @@@@The results showed that , after 40 days treatment through hyperlipidic diet , the obesity model group was successfully built up , as they were overweighed ( P < 0 .01 ) and showed a higher mass of adipose tissue ( P <0 .05) , TC ( P< 0 .05) , TG ( P < 0 .01) contents than the normal diet control group . Compared with obesity model group , YCE exhibited an inhibitory effect on body mass to some extent ( P < 0 .01) , fat tissues , body fat percentage ( P < 0 .01) and Lee s index declined ( P < 0 .05) . The contents of TC , TG and LDL of the serum in YCE treatment groups were declined ( P< 0 .01) , while there was no difference in HDL level . The liver mass ( P<0 .01) and its TG contents ( P < 0 .05) in YCE treatment groups were lower than that of obesity model group . Meanwhile , TC content exhibited no significant change , on the other hand , the AST and ALT levels in the liver were normal , it showed that the YCE treatment had no side‐effect on liver tissues . The PL level in YCE treatment groups was decreased ( P < 0 .05) and it indicated that YCE might act on PL to affect lipidolysis . @@@@From the above tests we can conclude that YCE has a significant anti‐obesity effect . The changes in blood and liver indices , like TC , TG and so on , reflects its intervention on lipids metabolism . Moreover , YCE has no side‐effect on liver tissues . Also YCE has a potential inhibitory effect on Pancrelipase ( PL) , which can be a potential target for anti‐obesity therapy . Our results provide a further research evidence and basis for YCE s deep exploration and development .