CAJ | 학술논문

目的研究妊娠合并特发性血小板减少性紫癜(ITP)患者外周血淋巴细胞数及其亚群的变化,探讨这一特殊微环境下适应性免疫的变化及其对临床的指导意义.方法仪器分析法测定血常规,淋巴细胞计数反应适应性免疫强弱, FCM法检测外周血淋巴细胞亚群,T、B细胞分别反应细胞、体液免疫活性,采用t检验对比两组间淋巴细胞数及其亚群变化.结果观察(妊娠合并ITP)组总淋巴细胞数与对照(ITP)组比较变化不明显;观察组CD3+细胞、CD4+细胞比对照组显著增加(P<0.001),观察组CD19+细胞(P<0.05)、NK细胞(P<0.001)比对照组显著减少,观察组CD8+T细胞与对照组比较差异无统计学意义(P>0.05).结论与单纯ITP患者相比,妊娠合并ITP患者适应性免疫功能变化,表现为细胞免疫更加活跃,体液免疫受到抑制;同时佐证和支持小剂量糖皮质激素对妊娠合并ITP的治疗.
목적연구임신합병특발성혈소판감소성자전(ITP)환자외주혈림파세포수급기아군적변화,탐토저일특수미배경하괄응성면역적변화급기대림상적지도의의.방법의기분석법측정혈상규,림파세포계수반응괄응성면역강약, FCM법검측외주혈림파세포아군,T、B세포분별반응세포、체액면역활성,채용t검험대비량조간림파세포수급기아군변화.결과관찰(임신합병ITP)조총림파세포수여대조(ITP)조비교변화불명현;관찰조CD3+세포、CD4+세포비대조조현저증가(P<0.001),관찰조CD19+세포(P<0.05)、NK세포(P<0.001)비대조조현저감소,관찰조CD8+T세포여대조조비교차이무통계학의의(P>0.05).결론여단순ITP환자상비,임신합병ITP환자괄응성면역공능변화,표현위세포면역경가활약,체액면역수도억제;동시좌증화지지소제량당피질격소대임신합병ITP적치료.
Objective To reveal changes of the adaptive immunity in ITP during pregnancy and its clinical significance by detecting the lymphocytes and their subsets. Methods With instrument method to test the blood RT&FCM to lymphocyte subsets. Results While the lymphocytes didn’t change significantly in the two groups,its subsets did another way;In the experimental group, the percentage of CD3+celland CD4+cell elevated significantly,the percentage of CD19+cell and NK cell decreased significantly,no significant change was found in CD8+cell. Conclusion The cellular immunity became more active,while the humoral immunity was restrained in ITP patients during pregnancy.This also supported the treatment of ITP during pregnancy with small dose of glucocorticoid.