CAJ | 학술논문

目的探讨移植谷氨酸脱羧酶(GAD)基因修饰的神经干细胞(Neural Stem Cells,NSCs)在创伤性颅脑损伤(TBI)大鼠中的作用.方法将40只雄性SD大鼠随机分成4组,每组10只.利用电穿孔转染大鼠NSCs,通过脑立体定向仪分别将GAD转基因NSCs(GAD+NSCs组)、NSCs(NSCs组)、磷酸盐缓冲液(PBS,PBS组)移植到TBI大鼠局部损伤灶附近,通过TUNEL法检测NSCs的凋亡情况,对照组大鼠不做处理,并进行神经损坏严重程度评分(neurological severity score,NSS)对移植后大鼠的神经系统行为和运动功能进行评估.结果 NSS结果显示GAD+NSCs组和NSCs组在第10、20、30天神经功能评分均低于PBS组(P<0.05);GAD+NSCs组在第10和20天神经功能评分低于NSCs组(P<0.05);在转基因NSCs移植7d后神经细胞凋亡数明显最少.结论转基因NSCs移植后合成GDA能够促进TBI大鼠神经功能的恢复.
목적탐토이식곡안산탈최매(GAD)기인수식적신경간세포(Neural Stem Cells,NSCs)재창상성로뇌손상(TBI)대서중적작용.방법장40지웅성SD대서수궤분성4조,매조10지.이용전천공전염대서NSCs,통과뇌입체정향의분별장GAD전기인NSCs(GAD+NSCs조)、NSCs(NSCs조)、린산염완충액(PBS,PBS조)이식도TBI대서국부손상조부근,통과TUNEL법검측NSCs적조망정황,대조조대서불주처리,병진행신경손배엄중정도평분(neurological severity score,NSS)대이식후대서적신경계통행위화운동공능진행평고.결과 NSS결과현시GAD+NSCs조화NSCs조재제10、20、30천신경공능평분균저우PBS조(P<0.05);GAD+NSCs조재제10화20천신경공능평분저우NSCs조(P<0.05);재전기인NSCs이식7d후신경세포조망수명현최소.결론전기인NSCs이식후합성GDA능구촉진TBI대서신경공능적회복.
Objective To investigate the effect of neural stem cel s (NSCs) modified by glutamic acid decarboxylase (GAD) on rat traumatic brain injury. Methods GAD-transfected neural stem cel s (GAD+NSCs group), wild neural stem cel s (NSCs group) and PBS (PBS group) were transplanted into rats with traumatic brain injury. Neurological function was assessed by neurological severity score (NSS) in al groups after transplantation. TUNEL was employed to observe the cel apoptosis. Results The values of NSS in GAD+NSCs and NSCs groups were significantly lower than that of PBS group at 10h, d20 and d30 after cel transplantation(P<0.05). The NSS scores in GAD+NSCs group were lower than those in NSCs group at d20 and d30(P<0.05). At each time point, the rate of cel apoptosis in transplantation group was lower than that in model group (P<0.05). Conclusion Neural stem cel s modified by GAD can protect neural function in rats with traumatic brain injury.