CAJ | 학술논문

目的研究雷公藤内酯醇(triptolide)对体外培养的胶质瘤U87细胞侵袭性的抑制作用,并探讨其作用分子机制. 方法使用MTT法检测雷公藤内酯醇对胶质瘤U87细胞增殖抑制作用, Transwell实验检测雷公藤内酯醇处理后细胞侵袭能力的变化,进一步通过Real-Time PCR和Western印迹法检测雷公藤内酯醇对U87胶质瘤细胞的组织蛋白酶B(Cathepsins B,CB)、基质金属蛋白酶9(MMP-9)表达的影响. 结果雷公藤内酯醇以剂量-效应方式抑制胶质瘤细胞增殖,同时可减弱U87胶质瘤细胞的侵袭能力,下调其CB、MMP-9表达水平. 结论在体外实验中雷公藤内酯醇抑制U87胶质瘤细胞侵袭性生长的机制与下调CB及MMP-9的表达,降低胶质瘤细胞水解细胞外基质的能力有关.
목적 연구뢰공등내지순(triptolide)대체외배양적효질류U87세포침습성적억제작용,병탐토기작용분자궤제. 방법 사용MTT법검측뢰공등내지순대효질류U87세포증식억제작용, Transwell실험검측뢰공등내지순처리후세포침습능력적변화,진일보통과Real-Time PCR화Western인적법검측뢰공등내지순대U87효질류세포적조직단백매B(Cathepsins B,CB)、기질금속단백매9(MMP-9)표체적영향. 결과 뢰공등내지순이제량-효응방식억제효질류세포증식,동시가감약U87효질류세포적침습능력,하조기CB、MMP-9표체수평. 결론 재체외실험중뢰공등내지순억제U87효질류세포침습성생장적궤제여하조CB급MMP-9적표체,강저효질류세포수해세포외기질적능력유관.
@@@@ Objective To study the anti-invation effect of triptolide for U87 glioma cells and its molecular mechanism. Methods MTT assay was used to determine the growth inhibition by triptolide in glioma U87 cells. Tumor invasion and migration were examined by Transwell Chamber assay. Real-Time PCR and Westerm b1ot was conducted to detect including the alteration of Cathepsins B (CB) and MMP-9. Results Triptolide could significantly inhibite glioma U87 cell proliferation, reduce the cell ability of invasion, down-regulating MMP-9 and CB expression level. Conclusion Triptolide could depress the invasion of glioma cells owing to down-regulating the expression level of MMP-9 and CB, reduce the ability of hydrolyzing extracellular matrix in vitro.