CAJ | 학술논문

左旋多巴是治疗帕金森病的首选药物,在临床中应用广泛.本文评述了近年来左旋多巴合成的相关研究进展,包括全化学合成、天然植物中提取、生物酶转化以及代谢工程策略.全化学合成左旋多巴由于步骤多而且不对称性合成,存在工艺复杂和环境污染等问题.植物提取制备左旋多巴产量低,受到资源限制.生物转化包括酪氨酸酚解酶、酪氨酸酶及转氨酶等催化苯类前体合成左旋多巴.提出随着合成生物学的发展,构建代谢工程微生物,发酵生产左旋多巴是一种高效、环境友好的新方法,具有良好的应用前景.
좌선다파시치료파금삼병적수선약물,재림상중응용엄범.본문평술료근년래좌선다파합성적상관연구진전,포괄전화학합성、천연식물중제취、생물매전화이급대사공정책략.전화학합성좌선다파유우보취다이차불대칭성합성,존재공예복잡화배경오염등문제.식물제취제비좌선다파산량저,수도자원한제.생물전화포괄락안산분해매、락안산매급전안매등최화분류전체합성좌선다파.제출수착합성생물학적발전,구건대사공정미생물,발효생산좌선다파시일충고효、배경우호적신방법,구유량호적응용전경.
L-dopa has been widely used in clinical treatment for Parkinson's disease. The methods for L-DOPA production,including total chemical synthesis,extraction from plants,enzyme bioconversion and metabolic engineering,were summarized in this review. The total chemical synthesis of L-dopa was complicate due to its asymmetric process and associated with certain environmental issues. Extracting L-dopa from plant suffered low yield and was limited by plant resource. Bioconversion of phenols to L-dopa has been catalyzed by enzymes tyrosine phenol lyase,tyrosinase and transaminase. It is suggested that with the development of synthetic biology,fermentation of L-dopa by constructing metabolically engineered microorganisms would be a new high efficient and environment-friendly method,which demonstrates a promising process.