首都医药
首都醫藥
수도의약
CAPITAL MEDICINE
2013年
8期
57-58
,共2页
蜂胶胶囊%HPLC%吡啶甲酸铬
蜂膠膠囊%HPLC%吡啶甲痠鉻
봉효효낭%HPLC%필정갑산락
Propolis Capsules%HPLC%Chromium Picolinate
目的建立保健品蜂胶胶囊中吡啶甲酸铬的含量测定方法的检验操作规程(SOP).方法色谱柱为phenomenex?C18(250mm×4.6mm,5μm),乙腈-磷酸盐缓冲液(磷酸氢二钾4.35g,磷酸二氢钾3.40g,加水1000mL溶解)(10∶90)为流动相,流速:1.0mL/min,检测波长为254nm,柱温:35℃,进样量:10μL.结果吡啶甲酸铬在2.0948~104.7424μg/mL范围内具有良好的线性关系,回归方程:Y=1.307×104x-2.512×103,r=0.9999.平均回收率为98.68%(n=9).结论采用高效液相色谱法测定蜂胶胶囊中吡啶甲酸铬含量的方法简便、准确可靠,可作为蜂胶胶囊的质量控制方法.
目的建立保健品蜂膠膠囊中吡啶甲痠鉻的含量測定方法的檢驗操作規程(SOP).方法色譜柱為phenomenex?C18(250mm×4.6mm,5μm),乙腈-燐痠鹽緩遲液(燐痠氫二鉀4.35g,燐痠二氫鉀3.40g,加水1000mL溶解)(10∶90)為流動相,流速:1.0mL/min,檢測波長為254nm,柱溫:35℃,進樣量:10μL.結果吡啶甲痠鉻在2.0948~104.7424μg/mL範圍內具有良好的線性關繫,迴歸方程:Y=1.307×104x-2.512×103,r=0.9999.平均迴收率為98.68%(n=9).結論採用高效液相色譜法測定蜂膠膠囊中吡啶甲痠鉻含量的方法簡便、準確可靠,可作為蜂膠膠囊的質量控製方法.
목적건립보건품봉효효낭중필정갑산락적함량측정방법적검험조작규정(SOP).방법색보주위phenomenex?C18(250mm×4.6mm,5μm),을정-린산염완충액(린산경이갑4.35g,린산이경갑3.40g,가수1000mL용해)(10∶90)위류동상,류속:1.0mL/min,검측파장위254nm,주온:35℃,진양량:10μL.결과필정갑산락재2.0948~104.7424μg/mL범위내구유량호적선성관계,회귀방정:Y=1.307×104x-2.512×103,r=0.9999.평균회수솔위98.68%(n=9).결론채용고효액상색보법측정봉효효낭중필정갑산락함량적방법간편、준학가고,가작위봉효효낭적질량공제방법.
@@@@Objective To established a SOP for the determination of chromium picolinate in propolis capsules.Methods The HPLC method was performed with a phenomenex? C18(250mm×4.6mm,5μm) column,and the mobile phase was acetonitrile-phosphate buffer (dipotassium dehydrogen phosphate 4.35g,potassium dehydrogen phosphate 3.40g,dissolved by 1000mL water) (10∶90);the detecting wavelength was 254nm;column temperature at 35℃,injection volume 10μL.Results The calibration curve of chromium picolinate was linear within the range of 2.0948~104.7424μg/mL,the calibration curve was Y=1.307×104x-2.512×103,r=0.9999;the average recovery was 98.68%(n=9).Conclusion The method is simple,with HPLC to graphy method for determination of the content of chromium picolinate in propolis capsule is accurate and reliable,the method can be used for the quality control method of Propolis capsules.
