CAJ | 학술논문

组织构建%皮肤组织构建%瘢痕%创伤愈合%皮肤%转化生长因子β1%转化生长因子β3%肉芽组织%成纤维细胞%巨噬细胞%细胞因子%国家自然科学基金組織構建%皮膚組織構建%瘢痕%創傷愈閤%皮膚%轉化生長因子β1%轉化生長因子β3%肉芽組織%成纖維細胞%巨噬細胞%細胞因子%國傢自然科學基金
조직구건%피부조직구건%반흔%창상유합%피부%전화생장인자β1%전화생장인자β3%육아조직%성섬유세포%거서세포%세포인자%국가자연과학기금
tissue construction%skin tissue construction%scar%wound healing%skin%transforming growth factor beta 1%transforming growth factor beta 3%granulation tissue%fibroblast%macrophage%cytokine%National Natural Science Foundation of China

背景:转化生长因子β在组织创伤修复中发挥核心和关键作用.目的:观察转化生长因子β1和转化生长因子β3在大鼠皮肤瘢痕性创伤愈合过程中表达量及表达部位的变化.方法:制备大鼠皮肤全层切伤模型,长度1.5-2.0 cm,深及筋膜层.于伤后0 h,12 h,1 d,2 d,3 d,4 d,5 d,6 d,7 d处死大鼠,取损伤部位皮肤,采用免疫组织化学染色检测各时间点转化生长因子β1和转化生长因子β3的表达,并进行定量分析.结果与结论:免疫组织化学染色显示,在创伤愈合的早期阶段(伤后1-5 d),转化生长因子β1和转化生长因子β3免疫阳性颗粒主要出现在上皮细胞、上皮基底层细胞胞浆、巨噬细胞等免疫细胞胞浆及肉芽组织中;随着创伤修复时间的持续,免疫阳性颗粒主要出现在真皮层的成纤维细胞及细胞外基质中.其中转化生长因子β1的表达在创伤后1-5 d最强,而转化生长因子β3在创伤后六七天时开始明显表达.可见在大鼠皮肤瘢痕性创伤愈合过程中,转化生长因子β1的表达先于转化生长因子β3,提示转化生长因子β1与胶原形成及创伤修复关系密切,而转化生长因子β3在愈合后期表达量有升高趋势,其可能与创伤后期的组织改建密切相关.
배경:전화생장인자β재조직창상수복중발휘핵심화관건작용.목적:관찰전화생장인자β1화전화생장인자β3재대서피부반흔성창상유합과정중표체량급표체부위적변화.방법:제비대서피부전층절상모형,장도1.5-2.0 cm,심급근막층.우상후0 h,12 h,1 d,2 d,3 d,4 d,5 d,6 d,7 d처사대서,취손상부위피부,채용면역조직화학염색검측각시간점전화생장인자β1화전화생장인자β3적표체,병진행정량분석.결과여결론:면역조직화학염색현시,재창상유합적조기계단(상후1-5 d),전화생장인자β1화전화생장인자β3면역양성과립주요출현재상피세포、상피기저층세포포장、거서세포등면역세포포장급육아조직중;수착창상수복시간적지속,면역양성과립주요출현재진피층적성섬유세포급세포외기질중.기중전화생장인자β1적표체재창상후1-5 d최강,이전화생장인자β3재창상후륙칠천시개시명현표체.가견재대서피부반흔성창상유합과정중,전화생장인자β1적표체선우전화생장인자β3,제시전화생장인자β1여효원형성급창상수복관계밀절,이전화생장인자β3재유합후기표체량유승고추세,기가능여창상후기적조직개건밀절상관.
@@@@BACKGROUND: Transforming growth factor β plays a key role in the repair of tissue injury. OBJECTIVE: To investigate the expression level and distribution of transforming growth factor β1 and transforming growth factor β3 during healing of skin scarred wound. METHODS: The ful-thickness incised wound model was established in rats, 1.5-2.0 cm long to the fascia layer. Immunohistochemistry was used to quantitatively analyze the expression level and position of transforming growth factor β1 and transforming growth factor β3 in rats at 0, and 12 hours, 1, 2, 3, 4, 5, 6, and 7 days post injury. RESULTS AND CONCLUSION: Results showed that the transforming growth factor β1 and transforming growth factor β3 positive particles mainly distributed in cytoplasm of epithelial cel s and epithelial basilar membrane, macrophage and granulation tissue in the early stage of wound healing (1-5 days post injury). With prolonged time, the positive particles were mainly found in fibroblast and extracel ular matrix. The expression level of transforming growth factor β1 was up-regulated intensively during 1-5 days after injury, and transforming growth factor β3 was significantly expressed since 6 and 7 days post injury. That is, transforming growth factor β1 was earlier expressed than transforming growth factor β3 during wound healing. Results showed a close relationship between transforming growth factor β1 and col agen formation, as wel as wound repair. The expression level of transforming growth factor β3 was increased in the later stage of wound healing, indicating that transforming growth factor β3 may be related to the remodeling of the wound.