CAJ | 학술논문

背景:腺苷酸环化酶Ⅷ涉及促进吗啡耐受、戒断和强化性能,对晚期长时程增强效应、长时程记忆和对应激的适应等可塑性变化中发挥重要的作用.目的:基于慢性吗啡依赖大鼠纳洛酮催瘾戒断建立的条件性位置厌恶动物模型,观察在条件性位置厌恶建立前后,与成瘾密切相关脑区伏隔核壳区内腺苷酸环化酶Ⅷ基因表达的适应性变化.方法:选用清洁级雄性S D大鼠,设模型组(吗啡+纳洛酮组)、吗啡+盐水组和盐水+纳洛酮组.模型组采用连续6.5 d慢性吗啡腹腔注射10 mg/kg,纳洛酮一次催瘾注射0.3 mg/kg,同时与条件性位置训练箱搭配建立大鼠条件性位置厌恶模型,对照组依模型组对照注射等体积生理盐水.在条件性位置厌恶建立前后,采用免疫组织化学方法检测伏隔核壳区内腺苷酸环化酶Ⅷ基因的表达水平.结果与结论:条件性位置厌恶建立前,3组腺苷酸环化酶Ⅷ在伏隔核壳区表达水平差异无显著性意义(F=4.651,P=0.052);条件性位置厌恶建立后,吗啡+纳洛酮组腺苷酸环化酶Ⅷ在伏隔核壳区(F=4.874, P=0.028)内表达水平显著高于吗啡+盐水组和盐水+纳洛酮组.结果提示,伏隔核壳区内腺苷酸环化酶Ⅷ水平可能是调节阿片类物质戒断所致厌恶动机的关键因子之一;腺苷酸环化酶Ⅷ基因表达水平的变化可能是条件性位置厌恶建立相关的神经适应性变化的重要分子基础之一.
배경:선감산배화매Ⅷ섭급촉진마배내수、계단화강화성능,대만기장시정증강효응、장시정기억화대응격적괄응등가소성변화중발휘중요적작용.목적:기우만성마배의뢰대서납락동최은계단건립적조건성위치염악동물모형,관찰재조건성위치염악건립전후,여성은밀절상관뇌구복격핵각구내선감산배화매Ⅷ기인표체적괄응성변화.방법:선용청길급웅성S D대서,설모형조(마배+납락동조)、마배+염수조화염수+납락동조.모형조채용련속6.5 d만성마배복강주사10 mg/kg,납락동일차최은주사0.3 mg/kg,동시여조건성위치훈련상탑배건립대서조건성위치염악모형,대조조의모형조대조주사등체적생리염수.재조건성위치염악건립전후,채용면역조직화학방법검측복격핵각구내선감산배화매Ⅷ기인적표체수평.결과여결론:조건성위치염악건립전,3조선감산배화매Ⅷ재복격핵각구표체수평차이무현저성의의(F=4.651,P=0.052);조건성위치염악건립후,마배+납락동조선감산배화매Ⅷ재복격핵각구(F=4.874, P=0.028)내표체수평현저고우마배+염수조화염수+납락동조.결과제시,복격핵각구내선감산배화매Ⅷ수평가능시조절아편류물질계단소치염악동궤적관건인자지일;선감산배화매Ⅷ기인표체수평적변화가능시조건성위치염악건립상관적신경괄응성변화적중요분자기출지일.
@@@@BACKGROUND: Adenylate cyclase Ⅷ is involved in the promotion of morphine tolerance, withdrawal and enhancement, and plays an important role in plastic changes, such as the advanced long-term enhancement effect, long-term memory and stress adaptation. OBJECTIVE: To explore the changes of adenylate cyclase Ⅷ gene expression in the shel of accumbens nuclei before and after development of chronic morphine-induced conditioned place aversion in a rat through naloxone reminder addiction withdrawal. METHODS: Clean grade Sprague Dawley rats were divided into three groups: morphine+naloxone group, morphine+saline group and saline+naloxone group. Rats in the former one group received intraperitoneal injection of 10 mg/kg morphine continuously for 6.5 days, and intraperitoneal injection of 0.3 mg/kg naloxone; then the conditioned place aversion model was established combined with the conditioned place training. Rats in the latter two groups were injected with the same dose of saline as the morphine+naloxone group. The adenylate cyclase Ⅷ gene expression in the shel of accumbens nuclei was detected with immunohistochemistry method before and after development of chronic morphine-induced conditioned place aversion model. RESULTS AND CONCLUSION: Before chronic morphine-induced conditioned place aversion model establishment, there was no significant difference of the adenylate cyclase Ⅷ gene expression in the shel of accumbens nuclei (F=4.651, P=0.052); after conditioned place aversion establishement, the adenylate cyclase Ⅷ gene expression in the shel of accumbens nuclei in the morphine+naloxone group was significantly higher than that in the morphine+saline group and saline+naloxone group (F=4.874, P=0.028). The results indicate that the changes of adenylate cyclase Ⅷ gene expression may be one of the important molecular underpinnings of the conditioned place aversion.