CAJ | 학술논문

组织构建%组织构建与生物活性因子%放射损伤%基因转染%血管内皮生长因子%转化生长因子β1%咬肌%免疫组化%血管新生%其他基金組織構建%組織構建與生物活性因子%放射損傷%基因轉染%血管內皮生長因子%轉化生長因子β1%咬肌%免疫組化%血管新生%其他基金
조직구건%조직구건여생물활성인자%방사손상%기인전염%혈관내피생장인자%전화생장인자β1%교기%면역조화%혈관신생%기타기금
tissue construction%tissue construction and bioactive factor%irradiation injury%gene transfection%vascular endothelial growth factor%transforming growth factor beta 1%masseter muscle%immunohistochemistry%angiogenesis%other grants-supported paper

背景:放射线对恶性肿瘤的复发起预防作用,但长期大剂量放射治疗会对周围正常组织造成损害,因此如何预防及治疗放射线对周围组织的损伤一直是关注的焦点.目的:观察放射治疗对大鼠咬肌组织的损伤情况及基因转染血管内皮生长因子对咬肌组织血管生成的影响.方法:用直线加速器对 Wistar 大鼠咬肌组织进行照射,总剂量40 Gy ,照射后将重组质粒pcDNA4-HisMax-C/血管内皮生长因子165及空质粒分别转染至大鼠咬肌区.于治疗结束后2周行转化生长因子β1、血管内皮生长因子蛋白免疫组化染色,检测两种蛋白的表达情况,同时在光镜下观察大鼠咬肌组织的病理变化.结果与结论:放疗损伤组大鼠转化生长因子β1的表达水平明显高于正常对照组,重组质粒基因转染后咬肌组织血管内皮生长因子蛋白表达水平明显高于放疗损伤组、空质粒转染组及正常对照组.提示转化生长因子β1可以促进损伤组织的修复;血管内皮生长因子基因转染可促进放射治疗后咬肌组织血管的生成,从而促进放疗损伤的修复.
배경:방사선대악성종류적복발기예방작용,단장기대제량방사치료회대주위정상조직조성손해,인차여하예방급치료방사선대주위조직적손상일직시관주적초점.목적:관찰방사치료대대서교기조직적손상정황급기인전염혈관내피생장인자대교기조직혈관생성적영향.방법:용직선가속기대 Wistar 대서교기조직진행조사,총제량40 Gy ,조사후장중조질립pcDNA4-HisMax-C/혈관내피생장인자165급공질립분별전염지대서교기구.우치료결속후2주행전화생장인자β1、혈관내피생장인자단백면역조화염색,검측량충단백적표체정황,동시재광경하관찰대서교기조직적병리변화.결과여결론:방료손상조대서전화생장인자β1적표체수평명현고우정상대조조,중조질립기인전염후교기조직혈관내피생장인자단백표체수평명현고우방료손상조、공질립전염조급정상대조조.제시전화생장인자β1가이촉진손상조직적수복;혈관내피생장인자기인전염가촉진방사치료후교기조직혈관적생성,종이촉진방료손상적수복.
@@@@BACKGROUND: Long-term high-dose radiation therapy is important to prevent the recurrence of cancer. But the radioactive rays can also make hurt to the normal tissues. So, researchers focus on the protection of the surrounding normal tissues in the radiotherapy. OBJECTIVE: To study the impact of radiation on the rat masseter muscle and the revasularization ability of the irradiated tissue by vascular endothelial growth factor gene transfection. METHODS: The Wistar rats were radiated by linear accelerator at a dose of 40 Gy. And then the irradiated muscle tissues were transfected with pcDNA4-HisMax-C/vascular endothelial growth factor 165 or empty plasmids. After 2 weeks of gene therapy, transforming growth factor β1 and vascular endothelial growth factor protein were determined using immunohistochemical staining, and the pathological change of the rat masseter muscles was observed under a light microscope. RESULTS AND CONCLUSION: Transforming growth factor β1 protein was highly expressed in the irradiated tissue than the control tissue. Vascular endothelial growth factor protein was significantly increased in the gene transfected group than the radiation group, empty plasmid DNA transfection group and normal control group. These findings indicate that transforming growth factor β1 can improve damaged tissue repair, and vascular endothelial growth factor gene therapy can resume the ability of revasularization of irradiated tissue, thereby promoting irradiated tissue repair.