中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
21期
3801-3808
,共8页
孟繁星%李放%叶超群%阴彦斌%高阳
孟繁星%李放%葉超群%陰彥斌%高暘
맹번성%리방%협초군%음언빈%고양
生物材料%组织工程骨材料%椎间盘退行性变%富血小板血浆%脂肪间充质干细胞%组织工程%髓核细胞%影像学观察
生物材料%組織工程骨材料%椎間盤退行性變%富血小闆血漿%脂肪間充質榦細胞%組織工程%髓覈細胞%影像學觀察
생물재료%조직공정골재료%추간반퇴행성변%부혈소판혈장%지방간충질간세포%조직공정%수핵세포%영상학관찰
biomaterials%tissue-engineered bone materials%disc degeneration%platelet-rich plasma%adipose-derived mesenchymal stem cells%tissue engineering%nucleus pulposus cells%imaging observation
背景:富含血小板的血浆凝胶作为三维支架使其中干细胞可以呈立体生长,同时富含血小板的血浆凝胶又释放大量生长因子,促进脂肪间充质干细胞增殖及分化.目的:探讨脂肪间充质干细胞-富含血小板的血浆凝胶复合体注入兔椎间盘退变模型后的修复作用.方法:取兔动脉血采用二次离心法制备自体富血小板血浆,取兔肩胛间区脂肪分离培养脂肪间充质干细胞,制备脂肪间充质干细胞-富含血小板的血浆凝胶复合体.新西兰大白兔随机分为对照组、模型组、富含血小板的血浆凝胶组和脂肪间充质干细胞-富含血小板的血浆凝胶复合体组,后3组以穿刺法制备椎间盘退变模型,退变模型制备完成2周后,富含血小板的血浆凝胶组和脂肪间充质干细胞-富含血小板的血浆凝胶复合体组分别对退变间盘中注射相应材料.结果与结论:兔椎间盘退变后,间隙明显降低,髓核信号明显降低,髓核内基质高,密度染色较深;而经富含血小板的血浆凝胶和脂肪间充质干细胞-富含血小板的血浆凝胶复合体治疗后,上述症状明显改善,且脂肪间充质干细胞-富含血小板的血浆凝胶复合体的治疗效果更好.提示对退变椎间盘内注射富含血小板的血浆凝胶支架及脂肪间充质干细胞-富含血小板的血浆凝胶复合体均有利于减少退变对椎间盘的影响,其中脂肪间充质干细胞-富含血小板的血浆凝胶复合体注射效果更为突出.
揹景:富含血小闆的血漿凝膠作為三維支架使其中榦細胞可以呈立體生長,同時富含血小闆的血漿凝膠又釋放大量生長因子,促進脂肪間充質榦細胞增殖及分化.目的:探討脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體註入兔椎間盤退變模型後的脩複作用.方法:取兔動脈血採用二次離心法製備自體富血小闆血漿,取兔肩胛間區脂肪分離培養脂肪間充質榦細胞,製備脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體.新西蘭大白兔隨機分為對照組、模型組、富含血小闆的血漿凝膠組和脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體組,後3組以穿刺法製備椎間盤退變模型,退變模型製備完成2週後,富含血小闆的血漿凝膠組和脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體組分彆對退變間盤中註射相應材料.結果與結論:兔椎間盤退變後,間隙明顯降低,髓覈信號明顯降低,髓覈內基質高,密度染色較深;而經富含血小闆的血漿凝膠和脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體治療後,上述癥狀明顯改善,且脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體的治療效果更好.提示對退變椎間盤內註射富含血小闆的血漿凝膠支架及脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體均有利于減少退變對椎間盤的影響,其中脂肪間充質榦細胞-富含血小闆的血漿凝膠複閤體註射效果更為突齣.
배경:부함혈소판적혈장응효작위삼유지가사기중간세포가이정입체생장,동시부함혈소판적혈장응효우석방대량생장인자,촉진지방간충질간세포증식급분화.목적:탐토지방간충질간세포-부함혈소판적혈장응효복합체주입토추간반퇴변모형후적수복작용.방법:취토동맥혈채용이차리심법제비자체부혈소판혈장,취토견갑간구지방분리배양지방간충질간세포,제비지방간충질간세포-부함혈소판적혈장응효복합체.신서란대백토수궤분위대조조、모형조、부함혈소판적혈장응효조화지방간충질간세포-부함혈소판적혈장응효복합체조,후3조이천자법제비추간반퇴변모형,퇴변모형제비완성2주후,부함혈소판적혈장응효조화지방간충질간세포-부함혈소판적혈장응효복합체조분별대퇴변간반중주사상응재료.결과여결론:토추간반퇴변후,간극명현강저,수핵신호명현강저,수핵내기질고,밀도염색교심;이경부함혈소판적혈장응효화지방간충질간세포-부함혈소판적혈장응효복합체치료후,상술증상명현개선,차지방간충질간세포-부함혈소판적혈장응효복합체적치료효과경호.제시대퇴변추간반내주사부함혈소판적혈장응효지가급지방간충질간세포-부함혈소판적혈장응효복합체균유리우감소퇴변대추간반적영향,기중지방간충질간세포-부함혈소판적혈장응효복합체주사효과경위돌출.
@@@@BACKGROUND: Platelet-rich plasma gel serves as a three-dimensional scaffold in which stem cel s can exhibit a three-dimensional growth. Meanwhile, platelet-rich plasma gel can release large amounts of growth factors to promote the proliferation and differentiation of adipose-derived mesenchymal stem cel s. OBJECTIVE: To explore the repair effect of injection of autologous platelet rich plasma-adipose derived mesenchymal stem cel s complex on rabbit intervertebral disc degeneration. METHODS: Rabbit arterial blood was extracted to prepare autologous platelet-rich plasma using secondary centrifugal method, and adipose-derived mesenchymal stem cel s were isolated from the fat tissue of rabbit scapular area. Then, adipose-derived mesenchymal stem cel s-platelet-rich plasma gel complex was prepared. New Zealand white rabbits were randomized into control group, model group, platelet-rich plasma gel group and complex group (adipose-derived mesenchymal stem cel s-platelet-rich plasma gel complex). Intervertebral disc degeneration model was made in the latter three groups using puncture method. At 2 weeks after modeling, corresponding materials were injected into the disc in the four groups, respectively. RESULTS AND CONCLUSION: After intervertebral disc degeneration in rabbits, the intervertebral gap was significantly reduced, the nucleus pulposus signal was obviously decreased, nucleus pulposus matrix appeared to have a high density and to be deeply stained. The symptoms above mentioned were improved significantly after intervention with the platelet-rich plasma gel or the complex of adipose-derived mesenchymal stem cel s-platelet-rich plasma gel, while the latter was better in curative effects. Injection of platelet-rich plasma gel and adipose-derived mesenchymal stem cel s-platelet-rich plasma gel complex can reduce the influence of disc degeneration, and the effect of adipose-derived mesenchymal stem cel s-platelet-rich plasma gel complex is more outstanding.