CAJ | 학술논문

生物材料%组织工程骨材料%生长因子%骨形态发生蛋白2%壳聚糖%羟基磷灰石%聚乳酸-羟基乙酸%骨支架材料%下颌骨缺损%成骨%骨钙素%其他基金生物材料%組織工程骨材料%生長因子%骨形態髮生蛋白2%殼聚糖%羥基燐灰石%聚乳痠-羥基乙痠%骨支架材料%下頜骨缺損%成骨%骨鈣素%其他基金
생물재료%조직공정골재료%생장인자%골형태발생단백2%각취당%간기린회석%취유산-간기을산%골지가재료%하합골결손%성골%골개소%기타기금
biomaterials%tissue-engineered bone materials%growth factor%bone morphogenetic protein 2%chitosan%hydroxyapatite%poly(lactic-co-glycolic acid)%bone scaffold materials%mandibular bone defects%ossification%osteocalcin%other grants-supported paper

背景:支架材料联合细胞因子构建组织工程骨不受血管化和细胞培养因素的限制,这种构建模式可能诱导出较大体积的实用型组织工程骨.目的:观察壳聚糖纳米微球/纳米羟基磷灰石/聚乳酸-羟基乙酸复合生长因子缓释支架修复犬下颌骨临界骨缺损的能力.方法:取杂种犬12条,制作双侧下颌骨临界骨缺损模型,一侧植入复合生长因子骨形态发生蛋白2、转化生长因子β1及血管内皮生长因子165的壳聚糖纳米微球/纳米羟基磷灰石/聚乳酸-羟基乙酸缓释支架(实验组),另一侧植入壳聚糖纳米微球/纳米羟基磷灰石/聚乳酸-羟基乙酸缓释支架(对照组),术后4,8,12周取下颌骨标本行X 射线、组织学及免疫组织化学检查.结果与结论:实验组术后不同时间点X射线灰度值及骨钙素积分吸光度值均高于对照组(P <0.05),表明复合生长因子的支架材料修复骨缺损的成骨能力优于未复合生长因子的支架材料.组织学观察结果显示,实验组术后不同时间点成骨时间及效果均优于对照组,表明复合生长因子骨形态发生蛋白2、转化生长因子β1及血管内皮生长因子165的壳聚糖纳米微球/纳米羟基磷灰石/聚乳酸-羟基乙酸缓释支架可更快更有效地促进骨缺损修复.
배경:지가재료연합세포인자구건조직공정골불수혈관화화세포배양인소적한제,저충구건모식가능유도출교대체적적실용형조직공정골.목적:관찰각취당납미미구/납미간기린회석/취유산-간기을산복합생장인자완석지가수복견하합골림계골결손적능력.방법:취잡충견12조,제작쌍측하합골림계골결손모형,일측식입복합생장인자골형태발생단백2、전화생장인자β1급혈관내피생장인자165적각취당납미미구/납미간기린회석/취유산-간기을산완석지가(실험조),령일측식입각취당납미미구/납미간기린회석/취유산-간기을산완석지가(대조조),술후4,8,12주취하합골표본행X 사선、조직학급면역조직화학검사.결과여결론:실험조술후불동시간점X사선회도치급골개소적분흡광도치균고우대조조(P <0.05),표명복합생장인자적지가재료수복골결손적성골능력우우미복합생장인자적지가재료.조직학관찰결과현시,실험조술후불동시간점성골시간급효과균우우대조조,표명복합생장인자골형태발생단백2、전화생장인자β1급혈관내피생장인자165적각취당납미미구/납미간기린회석/취유산-간기을산완석지가가경쾌경유효지촉진골결손수복.
@@@@BACKGROUND: Scaffold materials are combined with cytokines to construct tissue-engineered bone, which cannot be limited by vascularization and cel culture. This reconstruction mode is able to induce a large-scale practical tissue-engineered bone. OBJECTIVE: To observe the ability of chitosan microsphere/nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffold with slow-released growth factors to repair critical mandibular defects in a dog. METHODS: Animal models of bilateral critical mandibular bone defects were established in 12 hybrid dogs. One side was implanted a chitosan microsphere/nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffold with bone morphogenetic protein 2, transforming growth factor-β1 and vascular endothelial growth factor 165 (experimental group); and the other side was implanted a chitosan microsphere/nano-hydroxyapatite/poly(lactic-co-glycolic acid). Manbidular specimens were harvested at postoperative weeks 4, 8 and 12 to carry out X-ray, histological and immunohistochemical examinations. RESULTS AND CONCLUSION: After surgery, the X-ray gray value and osteocalcin integral absorbance value in the experimental group were higher than those in the control group at different time points (P < 0.05), showing that the osteogenic ability of the compound growth factor scaffold is superior to the scaffold without growth factor in the repair of bone defects. Histological observation results showed that the ossification time and effect in the experimental group were better than those in the control group at different time point after surgery, indicating that the chitosan microsphere/nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffold with bone morphogenetic protein 2, transforming growth factor-β1 and vascular endothelial growth factor 165 can be faster and more effective to promote bone defect repair.