中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
21期
3839-3845
,共7页
生物材料%组织工程骨材料%浓缩红骨髓%富血小板纤维蛋白%纳米羟基磷灰石%骨%颌骨缺损%自体骨膜
生物材料%組織工程骨材料%濃縮紅骨髓%富血小闆纖維蛋白%納米羥基燐灰石%骨%頜骨缺損%自體骨膜
생물재료%조직공정골재료%농축홍골수%부혈소판섬유단백%납미간기린회석%골%합골결손%자체골막
biomaterials%tissue-engineered bone materials%concentrated red bone marrow%platelet-rich fibrin%nano-hydroxyapatite%bone%mandibular defects%autologous periosteum
背景:富血小板纤维蛋白支架结构有利于红骨髓中间充质干细胞及各种生长因子的生长,促进最终成骨.目的:探讨浓缩红骨髓/富血小板纤维蛋白复合载自体骨膜碎片支架材料修复兔下颌骨缺损的可行性.方法:制备新西兰大白兔双侧下颌骨人工制备骨缺损模型,采用自身对照方法,左侧为实验侧,植入自体浓缩红骨髓/富血小板纤维蛋白复合载自体骨膜碎片与纳米羟基磷灰石支架材料;右侧为对照侧,植入自体骨膜碎片复合纳米羟基磷灰石支架材料.术后2,4,8,12周制备组织标本,行大体观察、影像学分析、苏木精-伊红染色、扫描电镜检测.结果与结论:影像学检查及组织学染色显示,实验侧骨缺损处愈合程度、成骨速度及质量明显优于对照侧;扫描电镜显示实验侧复合材料与组织相容性好,无炎症刺激反应;牙CT分析数据及新骨形成情况分别证明实验侧骨密度CT值显著高于对照侧(P <0.05),实验侧新生骨面积显著高于对照侧(P <0.05).表明浓缩红骨髓/富血小板纤维蛋白复合载自体骨膜碎片支架材料具有明显骨诱导作用,可望成为临床应用中修复颌骨缺损的新型材料.
揹景:富血小闆纖維蛋白支架結構有利于紅骨髓中間充質榦細胞及各種生長因子的生長,促進最終成骨.目的:探討濃縮紅骨髓/富血小闆纖維蛋白複閤載自體骨膜碎片支架材料脩複兔下頜骨缺損的可行性.方法:製備新西蘭大白兔雙側下頜骨人工製備骨缺損模型,採用自身對照方法,左側為實驗側,植入自體濃縮紅骨髓/富血小闆纖維蛋白複閤載自體骨膜碎片與納米羥基燐灰石支架材料;右側為對照側,植入自體骨膜碎片複閤納米羥基燐灰石支架材料.術後2,4,8,12週製備組織標本,行大體觀察、影像學分析、囌木精-伊紅染色、掃描電鏡檢測.結果與結論:影像學檢查及組織學染色顯示,實驗側骨缺損處愈閤程度、成骨速度及質量明顯優于對照側;掃描電鏡顯示實驗側複閤材料與組織相容性好,無炎癥刺激反應;牙CT分析數據及新骨形成情況分彆證明實驗側骨密度CT值顯著高于對照側(P <0.05),實驗側新生骨麵積顯著高于對照側(P <0.05).錶明濃縮紅骨髓/富血小闆纖維蛋白複閤載自體骨膜碎片支架材料具有明顯骨誘導作用,可望成為臨床應用中脩複頜骨缺損的新型材料.
배경:부혈소판섬유단백지가결구유리우홍골수중간충질간세포급각충생장인자적생장,촉진최종성골.목적:탐토농축홍골수/부혈소판섬유단백복합재자체골막쇄편지가재료수복토하합골결손적가행성.방법:제비신서란대백토쌍측하합골인공제비골결손모형,채용자신대조방법,좌측위실험측,식입자체농축홍골수/부혈소판섬유단백복합재자체골막쇄편여납미간기린회석지가재료;우측위대조측,식입자체골막쇄편복합납미간기린회석지가재료.술후2,4,8,12주제비조직표본,행대체관찰、영상학분석、소목정-이홍염색、소묘전경검측.결과여결론:영상학검사급조직학염색현시,실험측골결손처유합정도、성골속도급질량명현우우대조측;소묘전경현시실험측복합재료여조직상용성호,무염증자격반응;아CT분석수거급신골형성정황분별증명실험측골밀도CT치현저고우대조측(P <0.05),실험측신생골면적현저고우대조측(P <0.05).표명농축홍골수/부혈소판섬유단백복합재자체골막쇄편지가재료구유명현골유도작용,가망성위림상응용중수복합골결손적신형재료.
@@@@BACKGROUND: The platelet-rich fibrin scaffold structure is conducive to the growth of red bone marrow mesenchymal stem cel s and various growth factors, promoting final osteogenesis. OBJECTIVE: To investigate the feasibility of the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffold for repair of rabbit mandibular defects. METHODS: Bilateral mandibular bone defect models were prepared in New Zealand white rabbits using self-control method. The left side was the experimental side, and implanted with the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds; the right side was the control side, and implanted with periosteum fragments and nano-hydroxyapatite composite scaffolds. Al experimental animals were sacrificed after 2, 4, 8, 12 weeks to prepare tissue samples for general observation, imaging analysis, hematoxylin-eosin staining and scanning electron microscope detection. RESULTS AND CONCLUSION: The imaging examination and histological staining showed that the experimental side was superior to the control side in the aspects of bone healing, osteogenesis speed and quality. Under the scanning electron microscope, the experimental side had better compatibility with surrounding tissues and no inflammation reaction. The data of the tooth CT and evidence of new bone formation showed the bone density and new bone area of the experimental side were significantly higher than that of the control side (P < 0.05). These data indicate that concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds has an obvious osteoinductive role, which is expected to be a new-type material for clinical repair of mandibular defects.
