CAJ | 학술논문

背景:纳米技术可改善脊髓组织工程生物材料的性能.目的:分析新型脊髓纳米组织工程支架的组织相容性.方法:以胶原为原料制备纤维定向排列及非定向排列的纳米纤维膜,培养及鉴定S D大鼠脊髓源性神经干细胞.将两种纳米纤维膜与SD乳鼠脊髓源性神经干细胞共培养,以正常培养的神经干细胞为对照,通过 MTT 实验检测纳米纤维膜的细胞相容性;以扫描电镜检测细胞在纳米纤维膜表面的黏附及增殖情况;将纳米纤维膜植入S D大鼠体内,通过组织学检查确定其降解情况及组织相容性;通过免疫组织化学实验确定神经干细胞在体内的存活及移动情况.结果与结论:两种纳米纤维膜表面的神经干细胞黏附及增殖情况良好,MT T实验结果表明纳米纤维膜的细胞相容性佳,电镜结果表明细胞在纳米纤维膜表面黏附良好,增殖情况佳;在体内纳米纤维膜降解情况良好,组织相容性佳;BrdU 标定的神经干细胞在 SD 大鼠体内存活并移动情况良好.结果表明新型纳米组织工程支架具有良好的细胞及组织相容性.
배경:납미기술가개선척수조직공정생물재료적성능.목적:분석신형척수납미조직공정지가적조직상용성.방법:이효원위원료제비섬유정향배렬급비정향배렬적납미섬유막,배양급감정S D대서척수원성신경간세포.장량충납미섬유막여SD유서척수원성신경간세포공배양,이정상배양적신경간세포위대조,통과 MTT 실험검측납미섬유막적세포상용성;이소묘전경검측세포재납미섬유막표면적점부급증식정황;장납미섬유막식입S D대서체내,통과조직학검사학정기강해정황급조직상용성;통과면역조직화학실험학정신경간세포재체내적존활급이동정황.결과여결론:량충납미섬유막표면적신경간세포점부급증식정황량호,MT T실험결과표명납미섬유막적세포상용성가,전경결과표명세포재납미섬유막표면점부량호,증식정황가;재체내납미섬유막강해정황량호,조직상용성가;BrdU 표정적신경간세포재 SD 대서체내존활병이동정황량호.결과표명신형납미조직공정지가구유량호적세포급조직상용성.
@@@@BACKGROUND: The properties of tissue engineering materials have been improved obviously with the rise and development of nanotechnology, the prospect of which is broad in spinal cord tissue engineering. OBJECTIVE: To observe the histocompatibility of new nano-scaffolds for spinal tissue engineering. METHODS: Electrospun aligned and randomly oriented nano fibrous scaffolds were made of collagen. Spinal cord derived neural progenitor cells from Sprague-Dawley rats were cultured and identified, and then the cells were cultured on the aligned and randomly oriented col agen nanofibrous scaffolds. Neural stem cells under normal culture served as controls. Scanning electron microscope was used to observe the cells’ adhesion and generation on the scaffold surface, and cel histocompatibility was determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Histocompatibility and degradation of nanofibrous scaffolds in vivo were determined through histological observation. The survival and movement of neural stem cells in the body were determined by immunohistochemistry experiments. RESULTS AND CONCLUSION: Superficial morphous of electrospun aligned and randomly oriented col agen nanofibrous scaffolds were in accordence with contrivable requisition. The cel adhesion and proliferation on the surface of the col agen nanofibrous scaffolds was perfect. Results from 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay showed that the cell ular compatibility of nano-scaffolds was good. Nanofibrous scaffolds degraded wel and their histocompatibility in vivo was good. The 5-bromo-2'-deoxyuridine-labeled cells in vivo survived and migrated wel . Histocompatibility of new nano-scaffolds for tissue engineering is satisfactory.