CAJ | 학술논문

生物材料%材料生物相容性%磷酸钙骨水泥%可注射%可降解%生物相容性%骨修复材料%热原反应%血液相容性%生物安全性%其他基金生物材料%材料生物相容性%燐痠鈣骨水泥%可註射%可降解%生物相容性%骨脩複材料%熱原反應%血液相容性%生物安全性%其他基金
생물재료%재료생물상용성%린산개골수니%가주사%가강해%생물상용성%골수복재료%열원반응%혈액상용성%생물안전성%기타기금
biomaterials%material biocompatibility%calcium phosphate cement%injectable%degradable%biocompatibility%osteoinductive materials%pyrogen reaction%hematocompatibility%biological safety%other grants-supported paper

背景:体外实验已证实新型磷酸钙骨水泥有良好的可注射性、力学性能、抗溃散性及体外降解性能.目的:验证新型可注射、可降解磷酸钙骨水泥的生物相容性.方法:①急性毒性实验:分别向昆明小鼠尾静脉可注射新型磷酸钙骨水泥浸提液与生理盐水.②热源实验:在新西兰兔耳缘静脉注射新型磷酸钙骨水泥浸提液.③溶血实验:在兔抗凝血分别加入新型磷酸钙骨水泥浸提液、生理盐水及双蒸水.④迟发型超敏反应实验:在豚鼠肩胛骨内侧部位分别注射可注射新型磷酸钙骨水泥浸提液与生理盐水,并进行敷贴激发实验.⑤体外细胞毒性实验:在L929系小鼠成纤维细胞株培养液中分别加入可注射新型磷酸钙骨水泥浸提液、聚乙烯浸提液及苯酚溶液.⑥微核实验:分别在昆明小鼠腹腔注射可注射新型磷酸钙骨水泥浸提液、生理盐水与环磷酰胺.⑦肌肉植入实验:将新型磷酸钙骨水泥植入新西兰兔脊柱两侧肌肉内.结果与结论:新型可注射磷酸钙骨水泥无毒,无刺激性及致敏性,无热源反应,具有良好的血液相容性,植入动物肌肉后为非组织刺激物,具有良好的生物相容性,因而具有较好的生物安全性.
배경:체외실험이증실신형린산개골수니유량호적가주사성、역학성능、항궤산성급체외강해성능.목적:험증신형가주사、가강해린산개골수니적생물상용성.방법:①급성독성실험:분별향곤명소서미정맥가주사신형린산개골수니침제액여생리염수.②열원실험:재신서란토이연정맥주사신형린산개골수니침제액.③용혈실험:재토항응혈분별가입신형린산개골수니침제액、생리염수급쌍증수.④지발형초민반응실험:재돈서견갑골내측부위분별주사가주사신형린산개골수니침제액여생리염수,병진행부첩격발실험.⑤체외세포독성실험:재L929계소서성섬유세포주배양액중분별가입가주사신형린산개골수니침제액、취을희침제액급분분용액.⑥미핵실험:분별재곤명소서복강주사가주사신형린산개골수니침제액、생리염수여배린선알.⑦기육식입실험:장신형린산개골수니식입신서란토척주량측기육내.결과여결론:신형가주사린산개골수니무독,무자격성급치민성,무열원반응,구유량호적혈액상용성,식입동물기육후위비조직자격물,구유량호적생물상용성,인이구유교호적생물안전성.
@@@@BACKGROUND: A novel calcium phosphate bone cement has been proved to have good injectability, biomechanical properties, anti-col apsibility and in vitro degradability. OBJECTIVE: To evaluate the biocompatibility of a novel injectable and degradable calcium phosphate cement. METHODS: (1) In acute toxicity test, cement extracts were applied to mice by tail vein injection. (25) In pyrogen test, extracts were applied to rabbits by mainline. (3) In hemolysis test, extracts were added in the fresh anticoagulant blood of rabbits. (4) In delayed-type hypersensitivity test, two steps, induced and aroused were applied to guinea pigs. (5) In cytotoxicity test in vitro, extracts of cement, polyethylene, and phenol were added in L929 cells to evaluate cytotoxicity. (6) In mammal micronucleus test, extracts, normal saline and cyclophosphamide were applied to mice by intraperitoneal injection. (7) In muscular implantation test, muscle specimens were histologically evaluated. RESULTS AND CONCLUSION: The cement-extracted liquid induced no acute toxicity in mice, no pyrogenic reaction in rabbits, no genetic toxicity in mice or allergic reaction in guinea pigs. This novel cement had a great biocompatibility and biological safety.