CAJ | 학술논문

背景:磁性复合骨水泥作为一种新型骨转移治疗材料,不仅能对骨缺损进行修复,还能在交变磁场下升温杀死骨肿瘤.目的:检测磁性复合骨水泥对小鼠L929成纤维细胞增殖活性的影响及细胞毒性反应.方法:采用CCK-8法检测含0%(即磷酸钙骨水泥组),10%,20% Fe3O4的磁性磷酸钙骨水泥浸提液及含0%(即聚甲基丙烯酸甲酯骨水泥组),10%,20%Fe3O4的聚甲基丙烯酸甲酯骨水泥浸提液对对数生长期小鼠L929成纤维细胞的毒性作用,每种浸提液又分为50%与100%两个浓度梯度;以正常培养基培养的小鼠L929成纤维细胞作为阴性对照组.结果与结论:①在100%浓度梯度下:除含20%Fe3O4的磁性聚甲基丙烯酸甲酯骨水泥组细胞数量较少、细胞形态异常外,其余磁性聚甲基丙烯酸甲酯骨水泥组细胞生长状态良好;含10%Fe3O4的磁性磷酸钙骨水泥组培养24 h时表现出轻度细胞毒性,毒性2级;含20%Fe3O4的聚甲基丙烯酸甲酯骨水泥在24-72 h为表现出轻、中度细胞毒性,毒性为2,3级.各磁性磷酸钙骨水泥组细胞数量及形态正常.②在50%浓度梯度下:各组细胞形态均正常,细胞毒性为0级或1级.表明磁性聚甲基丙烯酸甲酯骨水泥具有轻、中度细胞毒性,磁性磷酸钙骨水泥细胞相容性良好,基本无细胞毒性.
배경:자성복합골수니작위일충신형골전이치료재료,불부능대골결손진행수복,환능재교변자장하승온살사골종류.목적:검측자성복합골수니대소서L929성섬유세포증식활성적영향급세포독성반응.방법:채용CCK-8법검측함0%(즉린산개골수니조),10%,20% Fe3O4적자성린산개골수니침제액급함0%(즉취갑기병희산갑지골수니조),10%,20%Fe3O4적취갑기병희산갑지골수니침제액대대수생장기소서L929성섬유세포적독성작용,매충침제액우분위50%여100%량개농도제도;이정상배양기배양적소서L929성섬유세포작위음성대조조.결과여결론:①재100%농도제도하:제함20%Fe3O4적자성취갑기병희산갑지골수니조세포수량교소、세포형태이상외,기여자성취갑기병희산갑지골수니조세포생장상태량호;함10%Fe3O4적자성린산개골수니조배양24 h시표현출경도세포독성,독성2급;함20%Fe3O4적취갑기병희산갑지골수니재24-72 h위표현출경、중도세포독성,독성위2,3급.각자성린산개골수니조세포수량급형태정상.②재50%농도제도하:각조세포형태균정상,세포독성위0급혹1급.표명자성취갑기병희산갑지골수니구유경、중도세포독성,자성린산개골수니세포상용성량호,기본무세포독성.
@@@@BACKGROUND: Magnetic bone cement has been used as a new kind of material for bone metastasis, and it can repair bone defects and cure bone tumors with temperature rising under an alternating magnetic field. OBJECTIVE: To test the magnetic bone cement effects on cytotoxicity and cell proliferation of mouse L929 cells. METHODS: The cytotoxicity of magnetic calcium phosphate cement and polymethylmethacrylate bone cement leaching liquors, respectively, containing 0, 10%, 20% Fe3O4, to L929 cells at logarithmic growth phase was detected using Cell Count Kit-8 assay. Two kinds of leaching liquors were prepared to two concentration gradients, 50% and 100%. Mouse L929 cells cultured in normal culture medium were considered as control group. RESULTS AND CONCLUSION: (1) Under 100% concentration gradient: Cell s grew wel in polymethyl methacrylate bone cement groups containing 0, 10% Fe3O4 except for 20% Fe3O4. Magnetic calcium phosphate cement containing 10% Fe3O4 showed mild cell toxicity, graded as 2, after culture for 24 hours. During 24- 72 hours, polymethylmethacrylate bone cement containing 20% Fe3O4 exhibited mild or moderate cell toxicity and the toxicity was graded as 2 or 3. (2) Under 50 concentration gradient: Cells grew wel in al groups. The cell toxicity was graded as 0 or 1. Magnetic polymethylmethacrylate bone cement has mild or moderate cell toxicity and magnetic calcium phosphate cement exhibits a good biocompatibility with no cell toxicity.