CAJ | 학술논문

生物材料%生物材料基础实验%牛磺酸%铜%抗菌%创面愈合%血管内皮生长因子A%金黄色葡萄球菌%感染%内皮细胞%成纤维细胞%瘢痕%国家自然科学基金生物材料%生物材料基礎實驗%牛磺痠%銅%抗菌%創麵愈閤%血管內皮生長因子A%金黃色葡萄毬菌%感染%內皮細胞%成纖維細胞%瘢痕%國傢自然科學基金
생물재료%생물재료기출실험%우광산%동%항균%창면유합%혈관내피생장인자A%금황색포도구균%감염%내피세포%성섬유세포%반흔%국가자연과학기금
biomaterials%basic experiments of biomaterials%taurine%copper%antibacterial%wound healing%vascular endothelial growth factor A%Staphylococcus aureus%infection%endothelial cells%fibroblasts%scar%National Natural Science Foundation of China

背景:铜具有广谱杀菌及耐药性强的特点,而且铜作为人体必需微量元素在创面愈合过程中发挥着重要作用.作者前期的实验得到了利于生物体吸收的牛磺酸铜有机化合物.目的:检测牛磺酸铜的抗菌活性及其对感染创面愈合过程中血管内皮生长因子A表达的影响.方法:①通过MTT比色法检测牛磺酸铜对金黄色葡萄球菌的最低抑菌浓度.②72只SD大鼠随机分为牛磺酸铜组与生理盐水组两组,于每只大鼠背部制作1个直径1.5 cm的圆形金黄色葡萄球菌感染创面.牛磺酸铜组创面应用最低抑菌浓度的牛磺酸铜溶液1 mL,生理盐水组创面应用生理盐水1 mL,隔日用药1次,直到创面完全愈合.结果与结论:①经测定牛磺酸铜对金黄色葡萄球菌的最低抗菌浓度为4 g/L.②用药后第7,10天,牛磺酸铜组创面愈合率明显高于生理盐水组(P <0.05);至第21天,两组创面都完全愈合.③组织学观察显示:用药后第7天,牛磺酸铜组肉芽组织更成熟;用药后第14天,牛磺酸铜组胶原纤维排列整齐,瘢痕窄,胶原内可见再生的毛囊和皮脂腺.④免疫组织化学检测创面血管内皮生长因子A的表达变化显示:牛磺酸铜组血管内皮生长因子A在用药后第3天表达明显高于生理盐水组(P <0.01),第7,10天,牛磺酸铜组血管内皮生长因子A表达量下降,但仍高于生理盐水组(P <0.05).其余时间点表达未见明显差异(P >0.05).证实感染创面外用牛磺酸铜药液可以发挥有效的杀菌作用,提高创面愈合率,促进大鼠感染创面血管内皮生长因子A的表达,从而提高创面的愈合质量.
배경:동구유엄보살균급내약성강적특점,이차동작위인체필수미량원소재창면유합과정중발휘착중요작용.작자전기적실험득도료리우생물체흡수적우광산동유궤화합물.목적:검측우광산동적항균활성급기대감염창면유합과정중혈관내피생장인자A표체적영향.방법:①통과MTT비색법검측우광산동대금황색포도구균적최저억균농도.②72지SD대서수궤분위우광산동조여생리염수조량조,우매지대서배부제작1개직경1.5 cm적원형금황색포도구균감염창면.우광산동조창면응용최저억균농도적우광산동용액1 mL,생리염수조창면응용생리염수1 mL,격일용약1차,직도창면완전유합.결과여결론:①경측정우광산동대금황색포도구균적최저항균농도위4 g/L.②용약후제7,10천,우광산동조창면유합솔명현고우생리염수조(P <0.05);지제21천,량조창면도완전유합.③조직학관찰현시:용약후제7천,우광산동조육아조직경성숙;용약후제14천,우광산동조효원섬유배렬정제,반흔착,효원내가견재생적모낭화피지선.④면역조직화학검측창면혈관내피생장인자A적표체변화현시:우광산동조혈관내피생장인자A재용약후제3천표체명현고우생리염수조(P <0.01),제7,10천,우광산동조혈관내피생장인자A표체량하강,단잉고우생리염수조(P <0.05).기여시간점표체미견명현차이(P >0.05).증실감염창면외용우광산동약액가이발휘유효적살균작용,제고창면유합솔,촉진대서감염창면혈관내피생장인자A적표체,종이제고창면적유합질량.
@@@@BACKGROUND: Copper has a broad spectrum bactericidal characterization and plays an important role in wound healing. Authors’ previous study has obtained copper-taurine organic compound which provides a better bioavailability to the human body. OBJECTIVE: To detect the antibacterial activity of copper-taurine and study its effect on vascular endothelial growth factor A expression during infected wound healing. METHODS: (1) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to examine the minimum inhibitory concentration of copper-taurine against Staphylococcus aureus. (2) Seventy-two Sprague-Dawley rats were randomly divided into copper-taurine and normal saline groups. A 1.5-cm Staphylococcus aureus infected wound was made on the back of rats. Copper-taurine group was topically treated with copper-taurine liquor 1 mL per wound, and normal saline group treated with normal saline 1 mL per wound. RESULTS AND CONCLUSION: (1) The minimum inhibitory concentration of the copper-taurine against Staphylococcus aureus was 4 g/L. (2) On days 7 and 10, the percent wound contraction of the copper-taurine group was higher than that of the normal saline group (P < 0.05). Wounds in two groups healed until day 21. (3) Histological observation: On days 7 and 10, in the copper-taurine group, the granulation was matured showing capil ary vertically oriented, robust fusiform fibrocytes and moderate wel-arranged col agen. (4) Immunohistochemical examination of vascular endothelial growth factor A expression: On day 3, the expression of vascular endothelial growth factor A was significantly higher in the copper-taurine group compared with the normal saline group (P < 0.01). On days 7 and 10, the copper-taurine group stil expressed higher vascular endothelial growth factor A than the normal saline group (P < 0.05). There was no difference at other time points (P > 0.05). In conclusion, topically applied copper-taurine on rat infected cutaneous wound can reduce the quantity of microbe, improve vascular endothelial growth factor A expression during wound healing, thus improving the quality of wound healing.